TY  - JOUR
N2  - Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected from diarrheic calves and workers in closed and non-closed beef buffalo farm. Zoonotic Giardia assemblage A was isolated from both diarrheic children and their close contact calves while the calves were infected by animal genotype assemblage E under low contact conditions. This was identified after the amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 20 children and 28 calves Giardia isolates using nested PCR. Fractionation of different isolated Giardia assemblage and identification of specific fraction versus anti-Giardia-IgG antibodies in infected humans or animals using EITB revealed that ?-1 giardin antigen with molecular weight ranged 29-34KDa. This specific immunogenic assemblage was fraction-related where it reacted specifically versus antibodies in sera of humans or animals infected by this assemblage and did not cross-react with giardin of other assemblage or with sera of non-infected cases. The results of the current study highlighted the use of EITB as a preliminary investigation before genotyping in the identification of zoonotic giardiasis.
VL  - 10
EP  - 428
SN  - 23224568 (ISSN)
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096980954&doi=10.36380%2fscil.2020.wvj52&partnerID=40&md5=8c1d3aa9a0a7db7c6acdbd1b74b8550f
Y1  - 2020/09/25/
SP  - 421
IS  - 3
JF  - World's Veterinary Journal
PB  - Scienceline Publication, Ltd
TI  - The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals
KW  - Buffalo calves
KW  -  Genotype
KW  -  Giardia
KW  -  Human
KW  -  Immunoblot
KW  -  Nested PCR
ID  - eprints508
A1  - Ramadan, R. M.
A1  - Khalifa, M. M.
A1  - Kamel, N. O.
A1  - Abdel-Wahab, A. M.
A1  - El-Bahy, M. M.
AV  - public
ER  -