<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals"^^ . "Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected from diarrheic calves and workers in closed and non-closed beef buffalo farm. Zoonotic Giardia assemblage A was isolated from both diarrheic children and their close contact calves while the calves were infected by animal genotype assemblage E under low contact conditions. This was identified after the amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 20 children and 28 calves Giardia isolates using nested PCR. Fractionation of different isolated Giardia assemblage and identification of specific fraction versus anti-Giardia-IgG antibodies in infected humans or animals using EITB revealed that α-1 giardin antigen with molecular weight ranged 29-34KDa. This specific immunogenic assemblage was fraction-related where it reacted specifically versus antibodies in sera of humans or animals infected by this assemblage and did not cross-react with giardin of other assemblage or with sera of non-infected cases. The results of the current study highlighted the use of EITB as a preliminary investigation before genotyping in the identification of zoonotic giardiasis."^^ . "2020-09-25" . . "10" . "3" . . "Scienceline Publication, Ltd"^^ . . . "World's Veterinary Journal"^^ . . . "23224568" . . . . . . . . . . . . . . . . . . . "R. M."^^ . "Ramadan"^^ . "R. M. Ramadan"^^ . . "M. M."^^ . "Khalifa"^^ . "M. M. Khalifa"^^ . . "A. M."^^ . "Abdel-Wahab"^^ . "A. M. Abdel-Wahab"^^ . . "N. O."^^ . "Kamel"^^ . "N. O. Kamel"^^ . . "M. M."^^ . "El-Bahy"^^ . "M. M. El-Bahy"^^ . . . . . . "The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals (Text)"^^ . . . . . "WVJ 10(3) 421-428, Sep 25, 2020.pdf"^^ . . . "The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #508 \n\n\n \n The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals\n \n\n" . "text/html" . . . "Q Science (General)"@en . . . "SF Animal culture"@en . .