relation: http://eprints.science-line.com/id/eprint/508/
title: The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals
creator: Ramadan, R. M.
creator: Khalifa, M. M.
creator: Kamel, N. O.
creator: Abdel-Wahab, A. M.
creator: El-Bahy, M. M.
subject: Q Science (General)
subject: SF Animal culture
description: Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected from diarrheic calves and workers in closed and non-closed beef buffalo farm. Zoonotic Giardia assemblage A was isolated from both diarrheic children and their close contact calves while the calves were infected by animal genotype assemblage E under low contact conditions. This was identified after the amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 20 children and 28 calves Giardia isolates using nested PCR. Fractionation of different isolated Giardia assemblage and identification of specific fraction versus anti-Giardia-IgG antibodies in infected humans or animals using EITB revealed that α-1 giardin antigen with molecular weight ranged 29-34KDa. This specific immunogenic assemblage was fraction-related where it reacted specifically versus antibodies in sera of humans or animals infected by this assemblage and did not cross-react with giardin of other assemblage or with sera of non-infected cases. The results of the current study highlighted the use of EITB as a preliminary investigation before genotyping in the identification of zoonotic giardiasis.
publisher: Scienceline Publication, Ltd
date: 2020-09-25
type: Article
type: PeerReviewed
format: text
language: en
identifier: http://eprints.science-line.com/id/eprint/508/1/WVJ%2010%283%29%20421-428%2C%20Sep%2025%2C%202020.pdf
identifier:    (2020)     The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals.   World's Veterinary Journal.  pp. 421-428.  ISSN 23224568 (ISSN)   
relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096980954&doi=10.36380%2fscil.2020.wvj52&partnerID=40&md5=8c1d3aa9a0a7db7c6acdbd1b74b8550f
relation: https://dx.doi.org/10.36380/scil.2020.wvj52