@article{eprints508,
           title = {The Use of Giardia immunogenic Protein Fraction to Distinguish Assemblages in Humans and Animals},
          author = {R. M. Ramadan and M. M. Khalifa and N. O. Kamel and A. M. Abdel-Wahab and M. M. El-Bahy},
          number = {3},
       publisher = {Scienceline Publication, Ltd},
         journal = {World's Veterinary Journal},
            year = {2020},
           month = {September},
          volume = {10},
           pages = {421--428},
        keywords = {Buffalo calves, Genotype, Giardia, Human, Immunoblot, Nested PCR},
             url = {http://eprints.science-line.com/id/eprint/508/},
        abstract = {Buffalo calves have a high adverse effect on public health, specifically zoonotic Giardiasis in Egypt. The current study was carried out to investigate the use of enzyme-linked immunoelectrotransfer blot technique (EITB) as a preliminary step for the diagnosis of giardiasis before genotyping. For this purpose, fecal and blood samples were collected from diarrheic calves and workers in closed and non-closed beef buffalo farm. Zoonotic Giardia assemblage A was isolated from both diarrheic children and their close contact calves while the calves were infected by animal genotype assemblage E under low contact conditions. This was identified after the amplification and sequencing of a 292 bp fragment of 16S-rRNA ribosomal unit from 20 children and 28 calves Giardia isolates using nested PCR. Fractionation of different isolated Giardia assemblage and identification of specific fraction versus anti-Giardia-IgG antibodies in infected humans or animals using EITB revealed that {\ensuremath{\alpha}}-1 giardin antigen with molecular weight ranged 29-34KDa. This specific immunogenic assemblage was fraction-related where it reacted specifically versus antibodies in sera of humans or animals infected by this assemblage and did not cross-react with giardin of other assemblage or with sera of non-infected cases. The results of the current study highlighted the use of EITB as a preliminary investigation before genotyping in the identification of zoonotic giardiasis.}
}