eprintid: 492
rev_number: 9
eprint_status: archive
userid: 5
dir: disk0/00/00/04/92
datestamp: 2022-05-04 23:32:30
lastmod: 2022-05-04 23:32:41
status_changed: 2022-05-04 23:32:30
type: article
metadata_visibility: show
creators_name: Nugroho, E. P.
creators_name: Setiyono, A.
creators_name: Hadi, U. K.
creators_name: Winarsih, W.
creators_name: Astuti, D.
creators_id: agusse@apps.ipb.ac.id
title: Detection of Coxiella burnetii (Query Fever) DNA by Nested-PCR in Beef Cattle from Ampel Slaughterhouse, Boyolali Regency, Middle Java, Indonesia
ispublished: pub
subjects: Q1
subjects: SF
divisions: j13
full_text_status: public
keywords: Beef cattle, Boyolali, Coxiella burnetii, Nested-PCR, Query fever case.
abstract: Coxiella burnetii (C. burnetii) is a Gram-negative and obligate intracellular bacterium that causes Query fever (Q fever). The aim of the present study was to detect C. burnetii in beef cattle from Ampel slaughterhouse at Boyolali Regency, Middle Java, Indonesia. Spleen, heart, liver, lung, and kidney samples were collected from 100 cattle and used for Nested-PCR (nPCR) with four types of primers (OMP1, OMP2, OMP3, and OMP4). Five stages of pooling extraction were performed on 100 individual samples. The nPCR amplified a 437 bp DNA fragment from the fifth pool on the sampled heart, lung, and spleen. Furthermore, 10 individual samples from the fifth pool were re-tested by nPCR to find out the number of positive individual samples. Of 10 samples, the obtained result indicated the presence of C. burnetii DNA in 7 samples, 6 from Simmental cattle and 1 from Ongole cattle. Therefore, it can be strongly suspected that there are 7 out of 100 local breed beef cattle positive of Q fever at Boyolali Regency, Middle Java, Indonesia.
date: 2021-06-25
publication: World's Veterinary Journal
volume: 11
number: 2
publisher: Scienceline Publication, Ltd
pagerange: 267-272
id_number: https://dx.doi.org/10.54203/scil.2021.wvj35
refereed: TRUE
issn: 23224568 (ISSN)
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85109447486&partnerID=40&md5=0dd142e16b4a90d536db870bb53f3121
j_index: scopus
citation:    (2021)     Detection of Coxiella burnetii (Query Fever) DNA by Nested-PCR in Beef Cattle from Ampel Slaughterhouse, Boyolali Regency, Middle Java, Indonesia.   World's Veterinary Journal.  pp. 267-272.  ISSN 23224568 (ISSN)   
document_url: http://eprints.science-line.com/id/eprint/492/1/WVJ%2011%282%29%20267-272%2C%20June%2025%2C%202021.pdf