TY  - JOUR
TI  - Characterization of pregnancy-associated glycoprotein as a biomarker of pregnancy in Etawa crossbred goat
SP  - 181
SN  - 23224568 (ISSN)
EP  - 186
KW  - Biomarkers
KW  -  Etawa crossbred
KW  -  PAG
KW  -  Pregnancy
AV  - public
A1  - Ningtyas, I. K.
A1  - Lestari, T. D.
A1  - Hermadi, H. A.
IS  - 3
PB  - Scienceline Publication, Ltd
JF  - World's Veterinary Journal
VL  - 9
Y1  - 2019/09/25/
ID  - eprints489
N2  - Pregnancy-Associated Glycoprotein (PAG) is secreted by the placenta, which is produced in mononucleate and binucleate trophoblast cells. The current research was conducted to find out a substance for diagnosing early pregnancy in Etawa crossbred goats. Six Etawa crossbred goats (not pregnant, three months pregnant and four months pregnant) were subjected in the present study from Livestock Government Institution Breeding in Singosari, Malang. The research methods consisted of sample collection, identification PAG with sodium dodecyl sulfate?polyacrylamide gel electrophoresis, the determination of concentration with Biuret method and specificity test with Western Blot assay. The obtained results showed that the molecular weight of PAG from pregnant Etawa crossbred goats was 55.85 kDa. The average concentrations of PAG in the goats of non-pregnant, three months pregnant, and four months pregnant were 1.83±2.98, 3.79±2.72 and 4.36±2.63, respectively. The results of the speci?city test with the Western Blot molecular revealed a molecular mass of PAG was 55 kDa. The findings of the present study demonstrated PAG in Etawa crossbred goats can be used as an indicator of pregnancy.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081903280&doi=10.36380%2fscil.2019.wvj23&partnerID=40&md5=6b90c90df60230e22fe113af4bb0a943
ER  -