eprintid: 484
rev_number: 10
eprint_status: archive
userid: 5
dir: disk0/00/00/04/84
datestamp: 2022-05-09 00:24:53
lastmod: 2022-05-09 00:28:15
status_changed: 2022-05-09 00:24:53
type: article
metadata_visibility: show
creators_name: Mufa, R. M. D.
creators_name: Lastuti, N. D. R.
creators_name: Legowo, D.
creators_name: Mufasirin, .
creators_id: nunukdyah53@gmail.com
title: Detection of Cryptosporidiosis in Dogs of Veterinary Clinics in Surabaya City Using Acid-Fast Staining and PCR
ispublished: pub
subjects: Q1
subjects: SF
divisions: j13
full_text_status: public
keywords: Acid-fast staining, Cryptosporidium spp., Dogs, PCR
abstract: The need for maintaining pets, such as dogs, is increasing along with the human population. When individuals keep dogs as their pets, they must be aware of disease transmission from dogs. One of the disease agents transmitted from pets to their owners is Cryptosporidium spp. causing cryptosporidiosis. The aim of the present study was to detect Cryptosporidium spp. infection in dogs through a fecal examination using the acid-fast staining method (Ziehl Neelsen) confirmed with the molecular examination of Polymerase Chain Reaction (PCR). Detection of Cryptosporidium sp. in feces of dogs was set up by using an acid-fast staining method. Positive results of the acid-fast staining were further confirmed using PCR. Polymerase Chain Reaction used primary AB210854 specific to the Cryptosporidium canis and S139-S141 genes which were specific primary for the Cryptosporidium parvum gene. Results of the acid-fast staining showed that 80% of the samples (40 samples from total samples) were infected with Cryptosporidium spp. Further detection using PCR showed that four samples were positive for Cryptosporidium canis infection, and two samples showed positive results of Cryptosporidium parvum infection. Dog samples were mostly infected with Cryptosporidium spp. including Cryptosporidium canis and Cryptosporidium parvum through a fecal examination using acid-fast staining and PCR.
date: 2021-12-25
publication: World's Veterinary Journal
volume: 11
number: 4
publisher: Scienceline Publication, Ltd
pagerange: 602-607
id_number: https://dx.doi.org/10.54203/scil.2021.wvj76
refereed: TRUE
issn: 23224568 (ISSN)
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123267113&doi=10.54203%2fscil.2021.wvj76&partnerID=40&md5=ace398da75f2513dc7ad3537a1869a40
j_index: scopus
citation:    (2021)     Detection of Cryptosporidiosis in Dogs of Veterinary Clinics in Surabaya City Using Acid-Fast Staining and PCR.   World's Veterinary Journal.  pp. 602-607.  ISSN 23224568 (ISSN)   
document_url: http://eprints.science-line.com/id/eprint/484/1/WVJ%2011%284%29%20602-607%2C%20December%2025%2C%202021.pdf