TY  - JOUR
ID  - eprints439
A1  - Ghanem, N.
A1  - Amin, A.
A1  - Saeed, A. M.
A1  - Abdelhamid, S. M.
A1  - El-Sayed, A.
A1  - Farid, O. A.
A1  - Dessouki, S. M.
A1  - Faheem, M. S.
SP  - 146
Y1  - 2020/06/25/
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087871871&doi=10.36380%2fscil.2020.wvj19&partnerID=40&md5=46bac5c206da1e743fbf0e3913d1ee3f
TI  - Effects of curcumin supplementation on viability and antioxidant capacity of buffalo granulosa cells under in vitro culture conditions
KW  - Antioxidant
KW  -  Buffalo
KW  -  Granulosa cells
KW  -  Oxidative stress
KW  -  Viability.
PB  - Scienceline Publication, Ltd
IS  - 2
N2  - The current study was conducted to investigate the possible protective effect of curcumin supplementation on buffalo granulosa cells (GCs) under in vitro culture condition. Buffalo ovaries were collected from local abattoir in physiological saline solution and transported directly to laboratory. Follicular fluid containing GCs and cumulus-oocyte-complexes were aspirated from antral follicles with diameter 2-8 mm. The collected GCs were seeded (Approximately 375,000 viable cells) in an 8-well culture plate containing tissue culture medium-199 (TCM-199) and kept at 37 蚓 in a humidified atmosphere of 5% CO2. The curcumin was supplemented to TCM media at levels of 1, 2.5, 5 and 10 ?M for 24 and 48 h at 37 蚓 or kept without treatment as control group. The viability of cells was determined using the trypan blue test. Intracellular reactive oxygen species (ROS) level was assessed by measuring the fluorescent intensity of 6-carboxy-2?,7?-dichlorodihydro fluorescein diacetate (H2DCFDA). In addition, mitochondrial activity of GCs was determined. The results of the present study indicated that the viability of GCs under culture conditions was significantly decreased in groups treated with 1, 2.5, 5 and 10 然 curcumin (86.0%, 86.26%, 83.0% and 74.0%, respectively) compared to control group (93.60 %). The two groups of granulosa cells cultured with 2.5 and 5 然 curcumin recorded greater level of mitochondrial activity than the groups cultured with 1 然 and 10 然 curcumin. Moreover, there was a significant increase in ROS level in group cultured with 10 然 curcumin, compared to control and other experimental groups. The enzyme activity of catalase (CAT), superoxide dismutase (SOD), glutathione (GSH) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) was increased after treating in vitro cultured granulosa cells with 5 然 of curcumin. However, the enzymatic activity of CAT, SOD, GSH and DPPH was declined significantly 48 h post-curcumin treatment. In conclusion, supplementation of curcumin at low concentration (2.5 然) for 24 h to in vitro cultured GCs improved intracellular metabolic activity and antioxidant protective system, whereas it could not sustain this action for 48 h. Moreover, supplementation of curcumin at high concentration and for long duration may negatively affect viability of GCs under in vitro culture condition via induction of oxidative stress.
JF  - World's Veterinary Journal
EP  - 159
VL  - 10
AV  - public
SN  - 23224568 (ISSN)
ER  -