TY  - JOUR
JF  - World's Veterinary Journal
AV  - public
SN  - 23224568 (ISSN)
PB  - Scienceline Publication, Ltd
SP  - 617
TI  - Identifying the Virulent Factors of Clostridium perfringens Locally Isolated from Different Species
A1  - El-Helw, H. A.
A1  - Taha, M. M.
A1  - El-Sergany, E. F.
A1  - Kotb, E. E. Z.
A1  - Hussein, A. S.
A1  - Abdalla, Y. A.
ID  - eprints415
VL  - 10
IS  - 4
Y1  - 2020/12/25/
KW  - Clostridium perfringens
KW  -  CPA gene
KW  -  CPE gene
KW  -  Net B gene
EP  - 624
N2  - Clostridium perfringens incriminated in many diseases among different species of animals due to its ability to produce many virulence factors. In the current study, 135 intestinal samples were collected from different animal species of different localities in Egypt. Samples were subjected to isolation and identification (morphologically and biochemically) for obtaining Clostridium perfringens isolates (n=26, 19.25%). The PCR was carried out to elucidate the virulence factors. It was indicated that all the 26 Clostridium perfringens isolates had CPA gene and Clostridium perfringens enterotoxin (CPE gene), whereas 23% of isolates of chicken and cattle intestinal samples contained CPA, Net B, and CPE genes as virulence factors. Consequently, those isolates are highly recommended to be used in the preparation of enterotoxemia and necrotic enteritis vaccines as they are more virulent strains.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099529832&doi=10.29252%2fscil.2020.wvj74&partnerID=40&md5=dca731a12085bcab3cf2c55c177d593c
ER  -