TY  - JOUR
TI  - Isolation and Molecular Detection of Mycoplasma gallisepticum in Commercial Layer Chickens in Sylhet, Bangladesh
SP  - 614
SN  - 23224568 (ISSN)
EP  - 620
KW  - Culture
KW  -  Layer chicken
KW  -  Mycoplasma gallisepticum
KW  -  PCR
AV  - public
A1  - Basit, M. S. I.
A1  - Mamun, M. A.
A1  - Rahman, M. M.
A1  - Noor, M.
IS  - 4
PB  - Scienceline Publication, Ltd
JF  - World's Veterinary Journal
VL  - 11
Y1  - 2021/12/25/
ID  - eprints383
N2  - Mycoplasma gallisepticum induced poultry diseases are associated with a huge economic crisis and have a considerable impact on the poultry industry worldwide. The aim of the current study was to isolate and perform molecular detection of MG circulating pathogenic strain in the commercial layer farms in the Sylhet district of Bangladesh. The entire study was conducted from January 2018 to January 2019 at three Upazilas of Sylhet district in Bangladesh. A total of 50?dead layer chickens (indicating signs of respiratory distress before death) were collected randomly from 15 different layer farms. The tissue samples, such as air sacs, trachea, and lungs, were taken from suspected dead chickens. Both cultural and PCR-based techniques were applied to identify Mycoplasma from tissue samples. The conventional PCR technique was implemented to amplify 185?bp DNA fragments for the MG. Out of 50 samples, 36% (18/50) and 70% (35/50) of MG were identified by cultural method and PCR, respectively. Based on the results of the study, it can be concluded that PCR is an easier, more sensitive, and less time-consuming method for the early diagnosis of MG in chickens, compared to cultural isolation and hence can lower the economic burden to poultry farmers caused by this disease.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123241727&doi=10.54203%2fscil.2021.wvj78&partnerID=40&md5=59ce9167bfb8feee545f479eca8bbc0f
ER  -