eprintid: 320
rev_number: 8
eprint_status: archive
userid: 3
dir: disk0/00/00/03/20
datestamp: 2022-05-23 18:25:27
lastmod: 2022-05-23 18:25:27
status_changed: 2022-05-23 18:25:27
type: article
metadata_visibility: show
creators_name: Shakal, M.
creators_name: Maher, M.
creators_name: Metwally, A. S.
creators_name: AbdelSabour, M. A.
creators_name: Madbbouly, Y.
creators_name: Safwat, G.
creators_id: shakal2000@gmail.com
title: Molecular Identification of a Velogenic Newcastle Disease Virus Strain Isolated from Egypt
ispublished: pub
subjects: Q1
subjects: SF
divisions: j10
full_text_status: public
keywords: Broilers, Newcastle Disease, Poultry industry, Velogenic
abstract: Newcastle Disease Virus (NDV) is still a major concern for the Egyptian poultry industry in spite of the mass vaccination programs implemented from a long years ago. The current study aimed to carry out the molecular identification of surface glycoprotein genes of NDV field strain isolated from the Giza governorate, Egypt. Tracheae were collected from 10 broilers NDV-vaccinated chicken flocks (at least three samples from each flock) suffering from mild to moderate respiratory symptoms; with mortalities varying from 10-40% during October 2019. Only five samples showed HA positive activity after propagation in specific pathogen-free embryonated chicken eggs and only one sample was positive for Avian avulavirus 1 by real-time reverse transcription-PCR. Sequencing for the cleavage site of the F protein gene of the positive isolate showed the typical known sequence of velogenic NDV strains (112RRQKRF117). Phylogenetic analysis of both F and HN genes showed high similarity and close relation to Chinese strains of Genotype VII and more specifically subtype VIId, suggesting the role of migratory wild birds in NDV evolution in Egypt. In conclusion, further epidemiological and surveillance studies are strongly recommended to define the exact role of migratory wild birds in NDV evolution in Egypt.
date: 2020-06-14
publication: Journal of World's Poultry Research
volume: 10
number: 2s
publisher: Scienceline Publication, Ltd
pagerange: 195-205
id_number: 10.36380/JWPR.2020.25
refereed: TRUE
issn: 2322455X (ISSN)
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099636879&doi=10.36380%2fJWPR.2020.25&partnerID=40&md5=0968ef81520cdef33ccb5cef7c80d359
j_index: scopus
citation:    (2020)     Molecular Identification of a Velogenic Newcastle Disease Virus Strain Isolated from Egypt.   Journal of World's Poultry Research.  pp. 195-205.  ISSN 2322455X (ISSN)   
document_url: http://eprints.science-line.com/id/eprint/320/1/JWPR%2010%282S%29%20195-202%2C%202020.pdf