TY  - JOUR
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075000513&doi=10.36380%2fjwpr.2019.16&partnerID=40&md5=fde18e25b6977b49905ddf17f3cf8384
SP  - 133
Y1  - 2019/09/25/
AV  - public
A1  - Salama, S. S.
A1  - Gadallah, F. M.
A1  - Abo-Elkhir, F. E. Z. G.
A1  - Khedr, A. A.
A1  - Ali, M. A.
KW  - Challenge and chicken
KW  -  ELISA
KW  -  Inactivated vaccine
KW  -  Pasteurella multocida
KW  -  Single dose
EP  - 138
VL  - 9
SN  - 2322455X (ISSN)
TI  - Uses of single dose dependent and relative potency assays for evaluation of inactivated Fowl Cholera Vaccine
N2  - Fowl cholera is a septicemic respiratory complex caused by Pasteurella multocida, widely distributed in poultry and other avian species and of major economic importance. A total of 37 different inactivated Pasteurella multocida vaccines from different sources either locally prepared or imported from different sources were comparatively tested for relative potency following both single dose and booster dose vaccination assays. The study objective was to minimize the time factor exhausted in the evaluation processes of the inactivated fowl cholera vaccines. So it is planned to compare between single and booster dose vaccinations and their related potency. Correlation between protection associated with the single dose and booster dose vaccination were evaluated and average requirement for protection was 43.7% in single dose vaccination assay compared to 76.2 % associated with booster dose vaccination assay. In the same concern, the correlation between both assays for the seroconversion was estimated using ELISA and the minimum requirement was 1.8× cut off value in the single dose vaccination assay compared to 2.25× cut off value in the booster dose vaccination assay. In conclusion, single dose vaccination assay could be valuable in the evaluation of inactivated fowl cholera vaccines through determination of protection indices and/or estimation of humoral immune response if the above mentioned data is considered.
PB  - Scienceline Publication, Ltd
JF  - Journal of World's Poultry Research
IS  - 3
ID  - eprints311
ER  -