eprintid: 303
rev_number: 8
eprint_status: archive
userid: 3
dir: disk0/00/00/03/03
datestamp: 2022-05-23 18:23:17
lastmod: 2022-05-23 18:23:17
status_changed: 2022-05-23 18:23:17
type: article
metadata_visibility: show
creators_name: Sabra, M.
creators_name: Abdellatif, W.
creators_name: Ahmed, A. I.
creators_name: Osman, N.
creators_id: nabila.osman@vet.svu.edu.eg
title: Molecular characterization and phylogenetic analysis of full-length S1 gene of GI-16 and GI-23 infectious bronchitis virus in Qena, Egypt
ispublished: pub
subjects: Q1
subjects: SF
divisions: j10
full_text_status: public
keywords: Full-length spike gene, GI-16, GI-23, Infectious bronchitis virus, Phylogenetic, RT-PCR.
abstract: Infectious Bronchitis Virus (IBV) is a highly evolving virus that affects respiratory, urinary and reproductive systems. This virus is recognized as an important pathogen due to the continuous genesis of new variants that threaten the poultry industry worldwide. The aim of this study was to characterize emerging IBV variants originated from field outbreaks in Qena province, Egypt, and to study their genetic relationships with global strains. From September 2017 to January 2019, 52 field samples were collected from broiler flocks suspected of being infected with IBV. The collected samples were inoculated into embryonated chicken eggs via allantoic route for virus isolation. The IBV presence was confirmed using real-time reverse transcriptase PCR (rRT-PCR) assay targeting nucleocapsid (N) gene and finally, nine samples were selected from 29 positive samples with rRT-PCR for further genetic characterization through full-length spike (S1) gene sequencing. Phylogenetic analyses indicated that one isolate (IBV/CK/EG/QENA-4/2017) clustered within genotype I lineage 16 (GI-16). On the other hand, the remaining eight isolates (2017-2018) belonged to genotype I lineage 23 (GI-23) and clustered separately in monophyletic clade. The isolates in this study were found to share only 74.6-82.1% amino acid identity with the commonly used vaccine strains in Egypt. In conclusion, findings of this study provide informative data on circulating IBVs in the study area and highlight the importance of adopting a convenient vaccination strategy that can be more efficient for controlling the emergence of new IBV variants.
date: 2020-03-25
publication: Journal of World's Poultry Research
volume: 10
number: 1
publisher: Scienceline Publication, Ltd
pagerange: 71-80
id_number: 10.36380/JWPR.2020.10
refereed: TRUE
issn: 2322455X (ISSN)
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086864722&doi=10.36380%2fJWPR.2020.10&partnerID=40&md5=8093ca57200d6826fc6d9787d0bb0c52
j_index: scopus
citation:    (2020)     Molecular characterization and phylogenetic analysis of full-length S1 gene of GI-16 and GI-23 infectious bronchitis virus in Qena, Egypt.   Journal of World's Poultry Research.  pp. 71-80.  ISSN 2322455X (ISSN)   
document_url: http://eprints.science-line.com/id/eprint/303/1/JWPR%2010%281%29%2071-80%2C%202020.pdf