TY  - JOUR
TI  - Antibacterial Sensitivity and Detection of Virulence Associated Gene of Pasteurella multocida Isolated from Rabbits
SP  - 165
SN  - 2322455X (ISSN)
EP  - 171
KW  - Antibacterial resístanse Pasteurella multocida
KW  -  toxA gene
KW  -  virulence genes
AV  - public
A1  - Mohamed, F. M.
A1  - Mansy, M. F.
A1  - Abd-Al-Jwad, A. E. T. M.
A1  - Hassan, A. K.
IS  - 2s
PB  - Scienceline Publication, Ltd
JF  - Journal of World's Poultry Research
VL  - 10
Y1  - 2020/06/14/
ID  - eprints254
N2  - The aim of the present work was to determine antibacterial sensitivity and resistance patterns of Pasteurella multocida isolated from rabbits in different farms of Assiut Governorate. Also, this study aimed to detect virulence-associated gene (toxA) of Pasteurella multocida. A total of 40 freshly dead rabbits were used to collect samples from liver, lung and subcutaneous abscess. In addition, tracheal swab samples were collected from 20 diseased rabbits. Bacteriological examination revealed that Pasteurella spp. were isolated and phenotypically identified with an incidence rate of 55% (33 out of 60 rabbits). Ten Pasteurella spp. isolates were randomly chosen for antibiotic sensitivity testing and molecular identification using PCR. Antibiotic sensitivity test was carried using standard disk diffusion method against 13 antibacterial drugs to determine antibacterial sensitivity and resistance patterns of Pasteurella isolates and revealed variable sensitivity and resistance to antibacterial drugs. Pasteurella multocida isolates were sensitive to wide variety of antibiotics (norfloxacin, enrofloxacin, ciprofloxacin, florfenicol, doxycycline, gentamycin, cephradine and cefoxitin). Three out of ten isolates were molecularly confirmed to be Pasteurella multocida and all of them demonstrated the presence of toxA virulence genes. In conclusion, the prevalence of Pasteurella infections in rabbits in Assiut Governorate was relatively high.
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099985582&doi=10.36380%2fJWPR.2020.21&partnerID=40&md5=a609ee9daccc1e74bc196f48268a8daa
ER  -