TY - JOUR UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086871186&doi=10.36380%2fJWPR.2019.26&partnerID=40&md5=4563add6ce82a84b8c8e24a75e0a1855 SP - 211 Y1 - 2019/12/25/ AV - public A1 - Mirnawati, . A1 - Ciptaan, G. A1 - Ferawati, . KW - Bacillus subtilis KW - Enzyme activity KW - Fermentation time KW - Inoculum doses KW - Palm Kernel Cake EP - 216 VL - 9 SN - 2322455X (ISSN) TI - The effect of Bacillus subtilis inoculum doses and fermentation time on enzyme activity of fermented palm kernel cake N2 - Palm kernel cake (PKC) was by-product of palm oil industry and it had potential to be one of the poultry ration ingredient However, its utilization for poultry was still limited because of the ?-mannan in PKC. In order to increase PKC utilization in poultry ration, fermentation process was done to remodeled ? mannan by using Bacillus subtilis. This research conducted a study on the effect of Bacillus subtilis inoculum dose and fermentation time to increase the enzyme activity of FPKC by using CRD with 3 × 3 factorial and 3 replications. Factor A was 3 doses of inoculum Bacillus subtilis: 3%, 5%, and 7%. Factor B was fermentation times which contained: (1) 2 days, (2) 4 days, and (3) 6 days. Parameters used were enzyme activity of mannanase, protease, and cellulase in FPKC. Significant interaction was seen between inoculum doses of Bacillus subtilis and fermentation time. There was also a significant interaction on each of the inoculums dose of Bacillus subtilis and fermentation time on all of the enzyme activity. This study concluded FPKC with Bacillus subtilis of 7% inoculums doses and 6 days fermentation time indicate the best result as seen from 24.27 U/ml of mannanase activity, 10.27 U/ml of protease activity, 17.13 U/ml of cellulose activity of fermented PKC. PB - Scienceline Publication, Ltd JF - Journal of World's Poultry Research IS - 4 ID - eprints253 ER -