eprintid: 211
rev_number: 8
eprint_status: archive
userid: 3
dir: disk0/00/00/02/11
datestamp: 2022-05-23 17:54:25
lastmod: 2022-05-23 17:54:25
status_changed: 2022-05-23 17:54:25
type: article
metadata_visibility: show
creators_name: Hasan Mohammed, M.
creators_name: Kandeil, A.
creators_name: Alkhazindar, M.
creators_name: Tarek AbdElSalam, E.
creators_name: Ahmed Ali, M.
creators_id: mohamedahmedali2004@yahoo.com
title: Isolation of Newcastle Disease Virus from Wild Migratory Birds in Egypt
ispublished: pub
subjects: Q1
subjects: SF
divisions: j10
full_text_status: public
keywords: Egypt, Fusion protein, Newcastle disease virus, Wild birds
abstract: Surveillance studies for Newcastle disease virus (NDV) are critical to monitor the potential spreading of these viruses among wild birds as well as domestic poultry. This study was conducted to determine the incidence of NDV in wild birds in Egypt in 2016. Out of 159 collected samples from eight different species of wild birds, six (3.77%) samples were positive for paramyxoviruses by semi-nested RT-PCR assay based on the RNA-dependent RNA polymerase gene. Of six positive samples, four NDVs were successfully isolated in 11-day-old specific-pathogen-free embryonated hens’ eggs. Partial sequences of the fusion gene of the four isolates were amplified using RT-PCR. Phylogenetic analysis of partial sequences of RNA-dependent RNA polymerase gene and fusion genes indicated that the detected NDV viruses in wild birds in Egypt are related to class I NDVs strains. Four Egyptian NDV isolates from wild birds exhibited sequence motif of 111GERQER↓LVG119 at the cleavage site as lentogenic virus in wild birds. Continuous active surveillance may help better monitoring of NDVs circulating in wild birds before newly emerging viruses in domestic poultry.
date: 2020-09-25
publication: Journal of World's Poultry Research
volume: 10
number: 3
publisher: Scienceline Publication, Ltd
pagerange: 520-526
id_number: 10.36380/jwpr.2020.60
refereed: TRUE
issn: 2322455X (ISSN)
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099616330&doi=10.36380%2fjwpr.2020.60&partnerID=40&md5=6c5b905802f2e2886a766c4791df7b64
j_index: scopus
citation:    (2020)     Isolation of Newcastle Disease Virus from Wild Migratory Birds in Egypt.   Journal of World's Poultry Research.  pp. 520-526.  ISSN 2322455X (ISSN)   
document_url: http://eprints.science-line.com/id/eprint/211/1/JWPR%2010%283%29%20520-526%2C%202020.pdf