TY  - JOUR
JF  - Journal of World's Poultry Research
KW  - Egypt
KW  -  Fusion protein
KW  -  Newcastle disease virus
KW  -  Wild birds
UR  - https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099616330&doi=10.36380%2fjwpr.2020.60&partnerID=40&md5=6c5b905802f2e2886a766c4791df7b64
VL  - 10
EP  - 526
PB  - Scienceline Publication, Ltd
IS  - 3
TI  - Isolation of Newcastle Disease Virus from Wild Migratory Birds in Egypt
Y1  - 2020/09/25/
SN  - 2322455X (ISSN)
N2  - Surveillance studies for Newcastle disease virus (NDV) are critical to monitor the potential spreading of these viruses among wild birds as well as domestic poultry. This study was conducted to determine the incidence of NDV in wild birds in Egypt in 2016. Out of 159 collected samples from eight different species of wild birds, six (3.77%) samples were positive for paramyxoviruses by semi-nested RT-PCR assay based on the RNA-dependent RNA polymerase gene. Of six positive samples, four NDVs were successfully isolated in 11-day-old specific-pathogen-free embryonated hens? eggs. Partial sequences of the fusion gene of the four isolates were amplified using RT-PCR. Phylogenetic analysis of partial sequences of RNA-dependent RNA polymerase gene and fusion genes indicated that the detected NDV viruses in wild birds in Egypt are related to class I NDVs strains. Four Egyptian NDV isolates from wild birds exhibited sequence motif of 111GERQER?LVG119 at the cleavage site as lentogenic virus in wild birds. Continuous active surveillance may help better monitoring of NDVs circulating in wild birds before newly emerging viruses in domestic poultry.
ID  - eprints211
AV  - public
A1  - Hasan Mohammed, M.
A1  - Kandeil, A.
A1  - Alkhazindar, M.
A1  - Tarek AbdElSalam, E.
A1  - Ahmed Ali, M.
SP  - 520
ER  -