eprintid: 1624
rev_number: 6
eprint_status: archive
userid: 3
dir: disk0/00/00/16/24
datestamp: 2025-12-26 08:22:14
lastmod: 2025-12-26 08:22:14
status_changed: 2025-12-26 08:22:14
type: article
metadata_visibility: show
creators_name: Ismail Saood, Alaa
creators_name: Azeez Khaleefa, Isam
creators_name: Nassir Aljassim, Khawla Bedan
creators_id: harith.najem@uobasrah.edu.iq
title: Isolation and Identification of Eimeria spp. From Domestic Pigeons (Columba livia domestica) in Basrah, Iraq
ispublished: pub
subjects: SF
divisions: j10
full_text_status: public
keywords: Eimeria chalcoptereae, Molecular technique, Phylogeny, Pigeon
abstract: Coccidiosis is a widespread disease among pigeons worldwide, resulting in significant economic losses due to mortality, morbidity, and reduced feed efficiency. The present study aimed to isolate a field strain of Eimeria spp. in domestic pigeons (Columba livia domestica) in Basra, Iraq. To ensure precise species identification, light microscopy techniques were combined with the advanced molecular method of polymerase chain reaction (PCR). Sixty fresh fecal samples were collected from pigeon lofts, ensuring minimal environmental disturbance and contamination. The flotation method was employed to analyze fecal samples. The present results indicated that 36 out of 60 samples contained coccidian oocysts, which represented an overall prevalence of 60%. The PCR technique was employed to amplify the 18S rRNA genes, which were subsequently utilized for sample detection. Six representative sequences were selected, registered, and deposited in the NCBI database (PV459631.1, PV459632.1, PV459633.1, PV459634.1, PV459635.1, and PV459636.1). The present findings were validated through molecular analysis, and six representative gene sequences were submitted to the National Centre for Biotechnology Information (NCBI) database. The current study revealed a high prevalence of coccidiosis, affecting 60% of domestic pigeons in Basra, highlighting its impact on their health. Moreover, this is the first report of Eimeria chalcoptereae in pigeons from Basra, Iraq
date: 2025-09-25
publication: Journal of World’s Poultry Research
volume: 15
number: 3
publisher: Scienceline Publication
pagerange: 332-337
id_number: 10.36380/jwpr.2025.32
refereed: TRUE
issn: 2322-455X
official_url: https://jwpr.science-line.com/attachments/article/86/JWPR15(3)332-337,2025.pdf
j_index: scopus
citation:    (2025)     Isolation and Identification of Eimeria spp. From Domestic Pigeons (Columba livia domestica) in Basrah, Iraq.   Journal of World’s Poultry Research.  pp. 332-337.  ISSN 2322-455X   
document_url: http://eprints.science-line.com/id/eprint/1624/1/JWPR15%283%29332-337%2C2025%20%281%29.pdf