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primary topic: Serological Assays of Brucellosis in Unvaccinated Sheep and Goat in Indonesia

Serological Assays of Brucellosis in Unvaccinated Sheep and Goat in Indonesia
type: AcademicArticle
type: Article
abstract: Brucellosis is recognized as one of the most widespread zoonotic diseases and is often overlooked, impacting livestock populations worldwide. Annual routine screenings utilize serological assays, such as the Rose Bengal test (RBT) and the complement fixation test (CFT), to identify the presence of brucellosis. The present study aimed to evaluate the diagnostic and discriminative performance of the CFT, the locally produced RBT antigen from Indonesia, the commercial RBT antigen (Pourquier), and the indirect enzyme-linked immunosorbent assay (ELISA) using sera from Indonesian goats and sheep. A total of 184 serum samples were collected, including 92 from goats and 92 from sheep. The samples were obtained through jugular venipuncture from animals on small-scale goat and sheep farms in Java Island, Indonesia. The results were analyzed to determine true prevalence (TP), accuracy, and cross-tabulation with parallel interpretation to assess the sensitivity and specificity of each test analysis. The TP of brucellosis in small ruminant serum samples using local RBT, Pourquier RBT, CFT, and indirect ELISA was 6.99%, 9.74%, 0%, and 14.78% in goats, and 6.92%, 20.34%, 0%, and 7.81% in sheep, respectively. Moderate Kappa interrater reliability was observed between the local and Pourquier RBTs for both goats (0.537) and sheep (0.440). The combined RBT sensitivity in goats and sheep was 27% and 20%, respectively, while the combined RBT specificity was 92% in goats and 80.5% in sheep. Using more than one diagnostic method is essential for screening and investigating brucellosis in goat and sheep populations. A combination of RBT and indirect ELISA tests is recommended to identify Brucella reactors among small ruminants in Indonesia.
date: 2025-09-30
volume: 15
issue: 3
is part of: World’s Veterinary Journal
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Serological Assays of Brucellosis in Unvaccinated Sheep and Goat in Indonesia (Text)
has version: Serological Assays of Brucellosis in Unvaccinated Sheep and Goat in Indonesia (Text)
has published: Serological Assays of Brucellosis in Unvaccinated Sheep and Goat in Indonesia (Text)
subject: SF Animal culture

Serological Assays of Brucellosis in Unvaccinated Sheep and Goat in Indonesia (Text)
type: Document
has file: WVJ15(3)710-719,2025 (1).pdf
has part: WVJ15(3)710-719,2025 (1).pdf

Scienceline Publication
type: Organization

World’s Veterinary Journal
type: Collection
issn: 23224568

Tri Anggraeni Kusumastuti
type: Person
given name: Tri Anggraeni
family name: Kusumastuti

Widagdo Sri Nugroho
type: Person
given name: Widagdo
family name: Sri Nugroho

Ani Setianingrum
type: Person
given name: Ani
family name: Setianingrum

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primary topic: Serological Assays of Brucellosis in Unvaccinated Sheep and Goat in Indonesia

SF Animal culture
type: Concept