eprintid: 1569
rev_number: 7
eprint_status: archive
userid: 5
dir: disk0/00/00/15/69
datestamp: 2025-12-26 09:42:29
lastmod: 2025-12-26 09:42:29
status_changed: 2025-12-26 09:42:29
type: article
metadata_visibility: show
creators_name: Selem, Eman
creators_name: Tohamy Elariny, Eman Youssif
creators_name: Elnabawy, Nabawy Mostafa
creators_id: eman.elsaid@zu.edu.eg
title: Antibacterial Activities of Rosmarinus officinalis Extract against Enterococcus faecalis and Enterococcus faecium
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: Antibiotic resistance, Enterococcus faecalis, Enterococcus faecium, Hospital-acquired infection, Natural antibacterial agent, PCR-based identification, Rosmarinus officinalis
abstract: Hospital-acquired infections are caused by multidrug-resistant Enterococcus (E.) species, especially E. faecalis and E. faecium, which are zoonotic bacteria and pose a significant public health challenge. This study aimed to identify multidrug-resistant Enterococcus isolates and evaluate the antibacterial activity of Rosmarinus officinalis (R. officinalis) extract against E. faecalis and E. faecium. Fifty urine and stool samples were collected from hospitalized patients. Four multidrug-resistant strains—E. faecalis 6E, 7B, 10D, and E. faecium 15E—were identified by polymerase chain reaction (PCR) with universal primers (8F and 1492R) targeting the 16S rDNA gene to confirm their identity as Enterococcus species. The antibacterial efficacy of R. officinalis extract was assessed using disk diffusion and minimum inhibitory concentration (MIC) determination. High-Performance Liquid Chromatography (HPLC) analysis was conducted to identify phenolic bioactive compounds in R. officinalis. The extract demonstrated significant antibacterial activity, with MICs of 25 mg/mL and 30 mg/mL for E. faecalis and E. faecium, respectively. HPLC revealed phenolic compounds such as rosmarinic acid, caffeic acid, and ferulic acid, likely contributing to the antimicrobial properties. The R. officinalis extract could be a promising natural antimicrobial agent against multidrug-resistant Enterococcus species. Compounds such as rosmarinic acid, caffeic acid, ferulic acid, apigenin-7-glucoside, syringic acid, and p-hydroxybenzoic acid show potential as safer, environmentally friendly alternatives to traditional antibiotics, aiding in the fight against rising antibiotic resistance.
date: 2025-06-25
publication: World’s Veterinary Journal
volume: 15
number: 2
publisher: Scienceline Publication
pagerange: 498-508
id_number: 10.54203/scil.2025.wvj50
refereed: TRUE
issn: 2322-4568
official_url: https://wvj.science-line.com/attachments/article/86/WVJ15(2)498-508,2025.pdf
j_index: scopus
citation:    (2025)     Antibacterial Activities of Rosmarinus officinalis Extract against Enterococcus faecalis and Enterococcus faecium.   World’s Veterinary Journal.  pp. 498-508.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/1569/1/WVJ15%282%29498-508%2C2025%20%281%29.pdf