<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Detection of Ancylostoma ceylanicum and Toxascaris leonina in Bengal Tigers (Panthera tigris) and Asian Black Bears (Ursus thibetanus) Captured at Hanoi Wildlife Rescue Center, Vietnam"^^ . "The Hanoi Wildlife Rescue Center (WRC) plays a crucial role in the rescue, care, and release of wild animals. The health of these animals was meticulously evaluated during their care and before their reintroduction into their natural habitat. An annual surveillance study of parasitic infections in wildlife at the Hanoi WRC was conducted to establish a scientific basis for developing preventive measures to manage the health of these animals. A total of 46 fresh fecal samples were opportunistically collected immediately following defecation using a shovel. These samples were obtained from 25 tigers (age: 10.8±3.6 years) and 21 bears (age: 11.2±4.6 years) captured at the Hanoi WRC in November 2024. Fecal samples were examined using sedimentation and centrifugal flotation methods to identify the presence of parasites. Subsequently, parasite eggs were collected using a micropipette for DNA extraction. Polymerase chain reaction and sequencing techniques were used to determine the parasite species. It was found that 20.0% of tigers (5 out of 25) and 23.8% of bears (5 out of 21) were infected with parasitic worms. The identification of these infections was achieved through the examination of the morphological characteristics of the eggs. This analysis identified the presence of trematode eggs, nematode eggs, including Strongyle type, and Toxascaris leonina. Molecular analysis further identified Strongyle eggs as belonging to Ancylostoma ceylanicum, which accounted for 12.0% of infections in tigers and 23.8% in bears. Additionally, Toxascaris leonina was detected exclusively in tigers, representing 12.0% of infections. Trematode eggs were found solely in tiger feces; however, molecular amplification was unsuccessful due to the insufficient number of eggs detected. The discovery of two zoonotic nematodes, A. ceylanicum and To. leonina in tigers and bears at the Hanoi (WRC) underscores the potential risk of nematode transmission from wildlife to humans and domestic animals in this area and its surrounding areas. The findings of this study will aid in the development of a prevention program aimed at controling gastrointestinal helminths in wild animals within the study region."^^ . "2025-06-25" . . "15" . "2" . . "Scienceline Publication"^^ . . . "World’s Veterinary Journal"^^ . . . "23224568" . . . . . . . . . . . . . "Duc Minh"^^ . "Nguyen"^^ . "Duc Minh Nguyen"^^ . . "Thi Hoang"^^ . "Yen Nguyen"^^ . "Thi Hoang Yen Nguyen"^^ . . "Thi Thu"^^ . "Hang Trinh"^^ . "Thi Thu Hang Trinh"^^ . . . . . . "Detection of Ancylostoma ceylanicum and Toxascaris leonina in Bengal Tigers (Panthera tigris) and Asian Black Bears (Ursus thibetanus) Captured at Hanoi Wildlife Rescue Center, Vietnam (Text)"^^ . . . . . "WVJ15(2)283-291,2025 (1).pdf"^^ . . "HTML Summary of #1548 \n\n\n \n Detection of Ancylostoma ceylanicum and Toxascaris leonina in Bengal Tigers (Panthera tigris) and Asian Black Bears (Ursus thibetanus) Captured at Hanoi Wildlife Rescue Center, Vietnam\n \n\n" . "text/html" . . . "SF Animal culture"@en . .