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Detection of Luteinizing Hormone Receptor in Holstein Friesian Dairy Cows Undergoing Repeat Breeding
The tropical climate in Indonesia will cause heat stress in imported cattle, such as Holstein Friesian dairy cows, which farmers in East Java Province widely keep. Heat stress can cause repeat breeding cases caused by failure in ovulation, which is greatly influenced by luteinizing hormones, whose receptors are found in the blood. This study aimed to detect luteinizing hormone (LH) gene receptors in the blood of dairy cows undergoing repeat breeding. This research was conducted from April to August 2023 using 4 randomly drawn 30 Holstein Friesian dairy cows with repeat breeder cases at the Wilis Farmer Village Unit cooperative. East Java. A ten-milliliter blood sample was taken from the jugular vein of each Holstein Friesian Dairy Cow and placed into a sterile tube. Deoxyribonucleic acid (DNA) extraction of luteinizing hormone gene receptors from the blood of Holstein Frisian dairy cows was done using a DNA extraction kit. DNA amplification of the receptor gene of the luteinizing hormone of Holstein Frisian dairy cows' blood was done using the PCR method, and DNA amplification was checked by 1% agarose electrophoresis. The results of the luteinizing hormone gene receptor from the blood of four Holstein Frisian dairy cows undergoing repeat breeding at the Tani Wilis Village Unit cooperative, East Java, were at 600 bp on gel electrophoresis. The LHR gene profile is potentially used as a marker to detect dairy cows with repeat breeding under heat stress.
Detection of Luteinizing Hormone Receptor in Holstein Friesian Dairy Cows Undergoing Repeat Breeding (Text)
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