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primary topic: Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri

Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri
type: AcademicArticle
type: Article
abstract: Antibiotic resistance remains a global concern, with up to 1.91 million deaths projected to occur due to resistant Gram-negative and Gram-positive bacteria by 2050. The study hence aimed to assess the antimicrobial activity of Ziziphus spina-christi leaf extracts in relation to specific bacterial strains and elucidate the molecular mechanisms to validate the in vitro findings. Ziziphus spina-christi leaf extracts were tested against Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Shigella flexneri (S. flexneri). The leaf powder was subjected to both aqueous and methanol-dichloromethane extraction. Phytochemical products were determined by Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry for water extract and methanol dichloromethane extract, respectively. The agar well diffusion method, broth microdilution, and minimum bactericidal concentration against three bacterial species, S. aureus, E. coli, and S. flexneri, were used to assess the antibacterial activity of extracts. The results have shown that both plant extract has a significant level of antibacterial activity at higher concentrations (400 mg/ml) against the gram-positive bacteria. In addition, the methanol-dichloromethane extract exhibited the highest antibacterial activity against Gram-negative bacteria (S. flexneri, and E. coli), conversely, the water extract demonstrated a lower activity against S. flexneri and E. coli, with inhibition zones of 15 ± 0 mm for both bacteria. At a lower concentration (100 mg/ml), the methanol-dichloromethane extract produced inhibition zones of 19.6 ± 0.5 mm against S. aureus, closely followed by S. flexneri and E. coli. The water extract exhibited high antibacterial activity against Gram-positive bacteria. However, exhibited reduced antibacterial activity against S. flexneri and E. coli, indicating a concentration-dependent antibacterial effect. Extraction methods were significantly different, with products generated from …
date: 2025-03-30
volume: 15
issue: 1
is part of: World’s Veterinary Journal
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri (Text)
has version: Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri (Text)
has published: Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri (Text)
subject: SF Animal culture

Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri (Text)
type: Document
has file: WVJ15(1) 143-161, 2025 (1).pdf
has part: WVJ15(1) 143-161, 2025 (1).pdf

Scienceline Publication
type: Organization

World’s Veterinary Journal
type: Collection
issn: 23224568

John Benjamin Ochieng
type: Person
given name: John
family name: Benjamin Ochieng

Hamasat Mohammed Musa
type: Person
given name: Hamasat
family name: Mohammed Musa

Eddy Okoth Odari
type: Person
given name: Eddy
family name: Okoth Odari

http://eprints.science-line.com/id/eprint/1532/
title: HTML Summary of #1532 <epc:when xmlns:epc="http://eprints.org/ep3/control" test="title">Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri</epc:when>
format: text/html
primary topic: Antimicrobial Activity of Ziziphus spina-christi against Staphylococcus aureus, Escherichia coli and Shigella flexneri

SF Animal culture
type: Concept