eprintid: 1528
rev_number: 7
eprint_status: archive
userid: 5
dir: disk0/00/00/15/28
datestamp: 2025-12-26 09:18:17
lastmod: 2025-12-26 09:18:17
status_changed: 2025-12-26 09:18:17
type: article
metadata_visibility: show
creators_name: Jafar Hasan, Baqer
creators_name: Hasan Al-Mutar, Hayder Aabd-alkareem
creators_name: Kadhum Taher, Jawad
creators_id: Baqerhikma.iq@gmail.com
title: Effects of Adding Natural Honey to Semen Extender on Ram Epididymal Sperm Quality
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: Epididymal sperm, Honey, Ram, Semen extender, Sperm motility
abstract: Numerous studies have indicated that male infertility is often associated with poor semen quality. The present study investigated the use of natural honey as an antioxidant and nutrient additive to semen extender to evaluate its effect on sperm parameters. A total of 16 fresh testes from sexually mature rams were collected for the investigation and immediately transported to the reproductive technology laboratory at the College of Veterinary Medicine, Baghdad University. For the first group, sperm was collected and diluted with an egg yolk extender mixed with 10% natural honey, while the second group consisted of epididymal sperm diluted with 0.9% normal saline. Epididymal fluid was collected and evaluated for both groups. The results showed significant differences in mean individual motility between the two groups after 48 and 72 hours, as determined by the comparison of proportions. Egg yolk plus honey diluent was significantly more effective than normal saline diluent in preserving sperm cell viability after 48 and 72 hours. The same finding applied to progressive motility; the egg yolk plus honey diluent was significantly more efficient than the normal saline diluent for the time frame after 48 and 72 hours, respectively. In conclusion, the findings demonstrated that the egg yolk extender supplemented with 10% honey was more effective in preserving ram sperm motility over time than normal saline. The addition of honey to the egg yolk extender improved the motility, the live-dead ratio, and the viability of the liquid storage of ram epididymal fluid. Furthermore, egg yolk plays a crucial role in protecting sperm from the detrimental effects of low temperatures.
date: 2025-03-30
publication: World’s Veterinary Journal
volume: 15
number: 1
publisher: Scienceline Publication
pagerange: 109-116
id_number: 10.54203/scil.2025.wvj13
refereed: TRUE
issn: 2322-4568
official_url: https://wvj.science-line.com/attachments/article/85/WVJ15(1)%20109-116,%202025.pdf
j_index: scopus
citation:    (2025)     Effects of Adding Natural Honey to Semen Extender on Ram Epididymal Sperm Quality.   World’s Veterinary Journal.  pp. 109-116.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/1528/1/WVJ15%281%29%20109-116%2C%202025%20%281%29.pdf