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primary topic: In Vivo Evaluation of a Polyethylene Glycol-Based Cryoprotectant during Cold Stress in a Rat Model

In Vivo Evaluation of a Polyethylene Glycol-Based Cryoprotectant during Cold Stress in a Rat Model
type: AcademicArticle
type: Article
abstract: Cold stress is an environmental factor that impacts the viability of animals and humans. This study aimed to determine the effectiveness of a cryoprotectant based on polyethylene glycol in reducing cold stress in laboratory rats. For the experiment, 30 outbred Wistar rats (5 weeks) with an average body weight of 55.1 ± 5.3 g were used. Three groups of animals were formed (10 rats per group). The first group served as a positive control, kept at a room temperature of +18 – +20 °С and received 0.1 ml of 0.9% NaCl solution. The second group, the negative control, was kept in critically low temperatures (+2–+4°С) and administered 0.1 ml of 0.9% NaCl solution per experimental animal. Rats of the third group were subjected to cold stress and received 0.1 ml of the experimental preparation. Cold stress in laboratory rats was created using a cooling thermostat. Observation for 14 days included monitoring the dynamics of changes in the live weight of animals (before the start of the experiment, and on days 7 and 14 of the observation) as well as biochemical and haematological blood indicators. Fecal samples were collected from the rectum to determine the qualitative and quantitative state of the intestinal microbiota. The survival level of animals that received the experimental drug within fourteen days was 80.0%, compared to only 40% in the untreated group. When using the experimental drug in laboratory animals, an increase in body weight was noted. The number of full-fledged Escherichia coli in rats that received the drug was 3.4 times higher than the indicator of the group of animals that was kept at a critically low temperature without the drug. The prolonged low temperature in control rats had a negative effect on the animal's body as evidenced by increased leukocyte counts and ALT levels, as well as decreased ALT/AST ratio, and total bilirubin. The use of an experimental polyethylene glycol-based preparation had a positive effect on the weight of rats, blood parameters…
date: 2025-03-30
volume: 15
issue: 1
is part of: World’s Veterinary Journal
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: In Vivo Evaluation of a Polyethylene Glycol-Based Cryoprotectant during Cold Stress in a Rat Model (Text)
has version: In Vivo Evaluation of a Polyethylene Glycol-Based Cryoprotectant during Cold Stress in a Rat Model (Text)
has published: In Vivo Evaluation of a Polyethylene Glycol-Based Cryoprotectant during Cold Stress in a Rat Model (Text)
subject: SF Animal culture

In Vivo Evaluation of a Polyethylene Glycol-Based Cryoprotectant during Cold Stress in a Rat Model (Text)
type: Document
has file: WVJ15(1)86-95, 2025 (1).pdf
has part: WVJ15(1)86-95, 2025 (1).pdf

Scienceline Publication
type: Organization

World’s Veterinary Journal
type: Collection
issn: 23224568

Volodymyr Zazharskyi
type: Person
given name: Volodymyr
family name: Zazharskyi

Olexyi Zaslavsky
type: Person
given name: Olexyi
family name: Zaslavsky

Olexandr Sosnicky
type: Person
given name: Olexandr
family name: Sosnicky

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primary topic: In Vivo Evaluation of a Polyethylene Glycol-Based Cryoprotectant during Cold Stress in a Rat Model

SF Animal culture
type: Concept