TY  - JOUR
SP  - 64
TI  - Detection of the Zygote arrest 1 Gene in Oocytes, Zygotes, and Embryos of Pesisir Cattle with the Addition of IGF-1 within the In Vitro Maturation Media
PB  - Scienceline Publication
SN  - 2322-4568
ID  - eprints1523
A1  - Lidya Pertiwi, Sedrisa
A1  - Afriyani, Tinda
A1  - Jaswandi, .
JF  - World?s Veterinary Journal
AV  - public
UR  - https://wvj.science-line.com/attachments/article/85/WVJ15(1)64-71,%202025.pdf
N2  - Zygote arrest 1 (ZAR 1) is a maternal gene that plays a crucial role in the oocyte-to-embryo transition. The present study aimed to investigate the presence or absence of the ZAR 1 gene in oocytes, zygotes, and embryos of Pesisir Cattle. Ovaries were collected from cattle at slaughterhouses, and oocytes were retrieved at the Biotechnology Laboratory. The collected oocytes were matured in a maturation medium supplemented with Insulin-like Growth Factor-1 (IGF-1) at a concentration of 10 µg/ml or without IGF-1 for 24 hours in a CO? incubator maintained at 38.5°C. Following maturation, the oocytes were fertilized for 18 hours, and the resulting embryos were cultured for 48 hours in a CO? incubator at 38.5°C. The samples were then subjected to PCR analysis. The amplification results revealed the presence of the ZAR 1 gene band at the target size of 228 bp in oocytes matured with and without IGF-1. A comparative analysis of oocytes and embryos showed differences in the gene bands, particularly in samples supplemented with IGF-1. These findings suggest that IGF-1 supplementation during oocyte maturation significantly influences ZAR 1 gene expression in embryos. The observed variations in ZAR 1 gene expression across the oocyte, zygote, and embryo stages highlight the gene's pivotal role in reprogramming post-fertilization and maintaining early embryonic development.
EP  - 71
VL  - 15
KW  - Embryo
KW  -  Insulin-like Growth Factor-1
KW  -  Oocyte
KW  -  Zygote
KW  -  Zygote arrest 1 gene
Y1  - 2025/03/30/
IS  - 1
ER  -