<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Detection of the Zygote arrest 1 Gene in Oocytes, Zygotes, and Embryos of Pesisir Cattle with the Addition of IGF-1 within the In Vitro Maturation Media"^^ . "Zygote arrest 1 (ZAR 1) is a maternal gene that plays a crucial role in the oocyte-to-embryo transition. The present study aimed to investigate the presence or absence of the ZAR 1 gene in oocytes, zygotes, and embryos of Pesisir Cattle. Ovaries were collected from cattle at slaughterhouses, and oocytes were retrieved at the Biotechnology Laboratory. The collected oocytes were matured in a maturation medium supplemented with Insulin-like Growth Factor-1 (IGF-1) at a concentration of 10 µg/ml or without IGF-1 for 24 hours in a CO₂ incubator maintained at 38.5°C. Following maturation, the oocytes were fertilized for 18 hours, and the resulting embryos were cultured for 48 hours in a CO₂ incubator at 38.5°C. The samples were then subjected to PCR analysis. The amplification results revealed the presence of the ZAR 1 gene band at the target size of 228 bp in oocytes matured with and without IGF-1. A comparative analysis of oocytes and embryos showed differences in the gene bands, particularly in samples supplemented with IGF-1. These findings suggest that IGF-1 supplementation during oocyte maturation significantly influences ZAR 1 gene expression in embryos. The observed variations in ZAR 1 gene expression across the oocyte, zygote, and embryo stages highlight the gene's pivotal role in reprogramming post-fertilization and maintaining early embryonic development."^^ . "2025-03-30" . . "15" . "1" . . "Scienceline Publication"^^ . . . "World’s Veterinary Journal"^^ . . . "23224568" . . . . . . . . . . . . . "Tinda"^^ . "Afriyani"^^ . "Tinda Afriyani"^^ . . "Sedrisa"^^ . "Lidya Pertiwi"^^ . "Sedrisa Lidya Pertiwi"^^ . . "."^^ . "Jaswandi"^^ . ". Jaswandi"^^ . . . . . . "Detection of the Zygote arrest 1 Gene in Oocytes, Zygotes, and Embryos of Pesisir Cattle with the Addition of IGF-1 within the In Vitro Maturation Media (Text)"^^ . . . . . "WVJ15(1)64-71, 2025 (1).pdf"^^ . . "HTML Summary of #1523 \n\n\n \n Detection of the Zygote arrest 1 Gene in Oocytes, Zygotes, and Embryos of Pesisir Cattle with the Addition of IGF-1 within the In Vitro Maturation Media\n \n\n" . "text/html" . . . "SF Animal culture"@en . .