relation: http://eprints.science-line.com/id/eprint/1523/
title: Detection of the Zygote arrest 1 Gene in Oocytes, Zygotes, and Embryos of Pesisir Cattle with the Addition of IGF-1 within the In Vitro Maturation Media
creator: Lidya Pertiwi, Sedrisa
creator: Afriyani, Tinda
creator: Jaswandi, .
subject: SF Animal culture
description: Zygote arrest 1 (ZAR 1) is a maternal gene that plays a crucial role in the oocyte-to-embryo transition. The present study aimed to investigate the presence or absence of the ZAR 1 gene in oocytes, zygotes, and embryos of Pesisir Cattle. Ovaries were collected from cattle at slaughterhouses, and oocytes were retrieved at the Biotechnology Laboratory. The collected oocytes were matured in a maturation medium supplemented with Insulin-like Growth Factor-1 (IGF-1) at a concentration of 10 µg/ml or without IGF-1 for 24 hours in a CO₂ incubator maintained at 38.5°C. Following maturation, the oocytes were fertilized for 18 hours, and the resulting embryos were cultured for 48 hours in a CO₂ incubator at 38.5°C. The samples were then subjected to PCR analysis. The amplification results revealed the presence of the ZAR 1 gene band at the target size of 228 bp in oocytes matured with and without IGF-1. A comparative analysis of oocytes and embryos showed differences in the gene bands, particularly in samples supplemented with IGF-1. These findings suggest that IGF-1 supplementation during oocyte maturation significantly influences ZAR 1 gene expression in embryos. The observed variations in ZAR 1 gene expression across the oocyte, zygote, and embryo stages highlight the gene's pivotal role in reprogramming post-fertilization and maintaining early embryonic development.
publisher: Scienceline Publication
date: 2025-03-30
type: Article
type: PeerReviewed
format: text
language: en
identifier: http://eprints.science-line.com/id/eprint/1523/1/WVJ15%281%2964-71%2C%202025%20%281%29.pdf
identifier:    (2025)     Detection of the Zygote arrest 1 Gene in Oocytes, Zygotes, and Embryos of Pesisir Cattle with the Addition of IGF-1 within the In Vitro Maturation Media.   World’s Veterinary Journal.  pp. 64-71.  ISSN 2322-4568   
relation: https://wvj.science-line.com/attachments/article/85/WVJ15(1)64-71,%202025.pdf
relation: 10.54203/scil.2025.wvj8