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primary topic: Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation

Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation
type: AcademicArticle
type: Article
abstract: The increasing resistance of helminths such as Ascaridia galli to conventional anthelmintics has necessitated the search for alternative treatments from natural sources. This study aimed to assess the anthelmintic properties of phenanthroindolizidine alkaloids derived from Tylophora indica leaves, a plant renowned for its medicinal value, including its traditional use in treating respiratory disorders, inflammation, and various infections. The leaves were subjected to sequential extraction with chloroform, ethanol, and water, which produced yields of 7.2, 17.8, and 11.2, respectively. Phytochemical analysis revealed that the ethanol extract was rich in bioactive compounds, including significant amounts of alkaloids, flavonoids, phenols, and terpenoids. Quantitative analysis confirmed the ethanol extract’s superiority, displaying the highest contents of phenolics (7.51 ± 0.62 mg/g), flavonoids (9.34 ± 1.63 mg/g), and alkaloids (17.65 ± 1.69 mg/g), underscoring its potential for various therapeutic applications. Further fractionation and High-Performance Liquid Chromatography (HPLC) purification isolated key phenanthroindolizidine alkaloids, including Tylophorinidine, Tylophorine, Septicine, Tylophorinol, and Antofine. Structural characterization via Nuclear Magnetic Resonance (NMR) and High-Resolution Mass Spectrometry (HRMS) validated these compounds. In vitro assays demonstrated significant dose-dependent anthelmintic activity against Ascaridia galli worms. Ethanol extracts exhibited the highest mortality rates, achieving 100 mortality within 24 hours at a concentration of 5 mg/mL. The mixture of all five alkaloids at 500 µg/mL showed a synergistic effect, leading to rapid and complete anthelmintic action. The egg embryonation assay further highlighted the efficacy of these alkaloids. The egg embryonation assay further demonstrated the potent efficacy of these alkaloids, with the mixture at 500 µg/mL inhibiting 92.67 of egg development, surpassing the positive cont…
date: 2025-03-25
volume: 15
issue: 1
is part of: World's Veterinary Journal
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation (Text)
has version: Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation (Text)
has published: Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation (Text)
subject: SF Animal culture

Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation (Text)
type: Document
has file: WVJ15(1)9-22, 2025 (1).pdf
has part: WVJ15(1)9-22, 2025 (1).pdf

Scienceline Publication
type: Organization

World's Veterinary Journal
type: Collection
issn: 23224568

Bethi Neeraja
type: Person
given name: Bethi
family name: Neeraja

Kathi Yadagiri
type: Person
given name: Kathi
family name: Yadagiri

http://eprints.science-line.com/id/eprint/1500/
title: HTML Summary of #1500 <epc:when xmlns:epc="http://eprints.org/ep3/control" test="title">Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation</epc:when>
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primary topic: Synergistic Effects of Phenanthroindolizidine Alkaloids from Tylophora indica Against Ascaridia galli: Phytochemical Characterization and In vitro Evaluation

SF Animal culture
type: Concept