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primary topic: Sequential culture of rumen fluid as a sustainable inoculant for in vitro ruminants feed evaluation

Sequential culture of rumen fluid as a sustainable inoculant for in vitro ruminants feed evaluation
type: AcademicArticle
type: Article
abstract: Rumen fluid plays a crucial role in in vitro studies for evaluating ruminant feed. Maintaining microbial activity in rumen fluid can serve as a breakthrough approach to reducing dependence on fresh rumen fluid collection by utilizing sequential culture techniques. This study aimed to assess the effectiveness of rumen microbial inoculants through sequential cultures with a 48-hour incubation period. A completely randomized design was applied with four treatments: K1 = Culture 1 (inoculant derived from fresh rumen fluid), K2 = Culture 2 (inoculant derived from Culture 1), K3 = Culture 3 (inoculant derived from Culture 2), and K4 = Culture 4 (inoculant derived from Culture 3). The test substrates included dwarf elephant grass and Indigofera zollingeriana leaves using analysis in vitro sequential cultures adapted from Tilley and Terry (1963) and the Consecutive Batch Culture (CBC) method. Parameters measured included rumen fermentation characteristics such as pH, ammonia nitrogen (N-NH₃) concentration, total volatile fatty acid (VFA) production, and dry matter digestibility. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s HSD (Honest Significant Difference) test. The results showed that the sequential culture process significantly affected in vitro rumen fermentation characteristics. The pH remained stable within the optimal range (6.67–6.78). Increased culture sequences enhanced N-NH₃ concentration, total VFA production, and dry matter digestibility. It can be concluded that rumen microbial inoculants remain effective up to the fourth sequential culture for in vitro evaluation of ruminant feeds.
date: 2025-09-30
volume: 15
issue: 5
is part of: Online Journal of Animal and Feed Research
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Sequential culture of rumen fluid as a sustainable inoculant for in vitro ruminants feed evaluation (Text)
has version: Sequential culture of rumen fluid as a sustainable inoculant for in vitro ruminants feed evaluation (Text)
has published: Sequential culture of rumen fluid as a sustainable inoculant for in vitro ruminants feed evaluation (Text)
subject: SF Animal culture

Sequential culture of rumen fluid as a sustainable inoculant for in vitro ruminants feed evaluation (Text)
type: Document
has file: OJAFR15(5)274-282,2025.pdf
has part: OJAFR15(5)274-282,2025.pdf

Online Journal of Animal and Feed Research
type: Organization

Online Journal of Animal and Feed Research
type: Collection
issn: 22287701

Syahriani SYAHRIR
type: Person
given name: Syahriani
family name: SYAHRIR

Ahmad RIFAI
type: Person
given name: Ahmad
family name: RIFAI

Asmuddin NATSIR
type: Person
given name: Asmuddin
family name: NATSIR

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primary topic: Sequential culture of rumen fluid as a sustainable inoculant for in vitro ruminants feed evaluation

SF Animal culture
type: Concept