eprintid: 1376
rev_number: 7
eprint_status: archive
userid: 2
source: Scopus
dir: disk0/00/00/13/76
datestamp: 2025-06-18 12:59:09
lastmod: 2025-06-18 12:59:09
status_changed: 2025-06-18 12:59:09
type: article
metadata_visibility: show
creators_name: Iryos, Annisa Rahma
creators_name: Mirnawati, Mirnawati
creators_name: Harnentis, Harnentis
creators_name: Srifani, Anifah
creators_name: Yanti, Gusri
title: EFFECTIVENESS OF Lactobacillus fermentum CMUL-54 AND Lactobacillus fermentum B978 AS PROBIOTIC CANDIDATES PRODUCING MANNANASE, CELLULASE AND PROTEASE ACTIVITIES FOR POULTRY
ispublished: pub
subjects: SF
divisions: j11
full_text_status: public
keywords: Enzyme activity; L. fermentum B978; Lactobacillus fermentum CMUL-54; Probiotics
abstract: The present research investigated the potential of Lactobacillus fermentum strains CMUL-54 and B978 as a probiotic candidates with mannanase, cellulase, and protease activities. The materials used in this research included L. fermentum CMUL-54, L. fermentum B978, MRS Broth containing oxgall, and various equipment and chemicals for analyzing probiotic candidates, mannanase, cellulase, and protease activities. This study utilized quantitative analysis conducted using a paired two-sample t-test with ten replications. The results revealed that L. fermentum CMUL-54 could be significantly (P<0.01) used as a probiotic candidate, showing resistance to temperatures of 42°C (9.9x109±0.71 CFU/ml), gastric pH (72.35±0.80), bile salt resistance (87.69±3.66), and hydrophobicity test to the intestine (92.40±0.30). Lactobacillus fermentum CMUL-54 also exhibited significant inhibitory zones against lactic acid bacteria (LAB) and pathogenic bacteria such as Escherichia coli (13.27±0.13mm), Salmonella enteritidis (13.91±0.13mm), Staphylococcus aureus (17.75±0.24mm), high activity mannanase (12.36±0.61U/ml), cellulase (12.42±0.24U/ml) and protease (11.30±0.08U/ml). It is concluded that L. fermentum CMUL-54 exhibited superior probiotic properties compared to L. fermentum B978, thus positioning it as a more promising candidate for improving broiler performance through enhanced digestion and overall health. © The Author(s) 2025
date: 2025-03-30
publication: Online Journal of Animal and Feed Research
volume: 15
number: 2
publisher: Scienceline Publication, Ltd
pagerange: 117-125
id_number: 10.51227/ojafr.2025.14
refereed: TRUE
issn: 2228-7701
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-105003449016&doi=10.51227%2fojafr.2025.14&partnerID=40&md5=d75e7f35168cb85bd3cd71ad3bcb8661
j_index: scopus
citation:    (2025)     EFFECTIVENESS OF Lactobacillus fermentum CMUL-54 AND Lactobacillus fermentum B978 AS PROBIOTIC CANDIDATES PRODUCING MANNANASE, CELLULASE AND PROTEASE ACTIVITIES FOR POULTRY.   Online Journal of Animal and Feed Research.  pp. 117-125.  ISSN 2228-7701   
document_url: http://eprints.science-line.com/id/eprint/1376/1/OJAFR15%282%29117-125%2C2025.pdf