relation: http://eprints.science-line.com/id/eprint/1376/ title: EFFECTIVENESS OF Lactobacillus fermentum CMUL-54 AND Lactobacillus fermentum B978 AS PROBIOTIC CANDIDATES PRODUCING MANNANASE, CELLULASE AND PROTEASE ACTIVITIES FOR POULTRY creator: Iryos, Annisa Rahma creator: Mirnawati, Mirnawati creator: Harnentis, Harnentis creator: Srifani, Anifah creator: Yanti, Gusri subject: SF Animal culture description: The present research investigated the potential of Lactobacillus fermentum strains CMUL-54 and B978 as a probiotic candidates with mannanase, cellulase, and protease activities. The materials used in this research included L. fermentum CMUL-54, L. fermentum B978, MRS Broth containing oxgall, and various equipment and chemicals for analyzing probiotic candidates, mannanase, cellulase, and protease activities. This study utilized quantitative analysis conducted using a paired two-sample t-test with ten replications. The results revealed that L. fermentum CMUL-54 could be significantly (P<0.01) used as a probiotic candidate, showing resistance to temperatures of 42°C (9.9x109±0.71 CFU/ml), gastric pH (72.35±0.80), bile salt resistance (87.69±3.66), and hydrophobicity test to the intestine (92.40±0.30). Lactobacillus fermentum CMUL-54 also exhibited significant inhibitory zones against lactic acid bacteria (LAB) and pathogenic bacteria such as Escherichia coli (13.27±0.13mm), Salmonella enteritidis (13.91±0.13mm), Staphylococcus aureus (17.75±0.24mm), high activity mannanase (12.36±0.61U/ml), cellulase (12.42±0.24U/ml) and protease (11.30±0.08U/ml). It is concluded that L. fermentum CMUL-54 exhibited superior probiotic properties compared to L. fermentum B978, thus positioning it as a more promising candidate for improving broiler performance through enhanced digestion and overall health. © The Author(s) 2025 publisher: Scienceline Publication, Ltd date: 2025-03-30 type: Article type: PeerReviewed format: text language: en identifier: http://eprints.science-line.com/id/eprint/1376/1/OJAFR15%282%29117-125%2C2025.pdf identifier: (2025) EFFECTIVENESS OF Lactobacillus fermentum CMUL-54 AND Lactobacillus fermentum B978 AS PROBIOTIC CANDIDATES PRODUCING MANNANASE, CELLULASE AND PROTEASE ACTIVITIES FOR POULTRY. Online Journal of Animal and Feed Research. pp. 117-125. ISSN 2228-7701 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-105003449016&doi=10.51227%2fojafr.2025.14&partnerID=40&md5=d75e7f35168cb85bd3cd71ad3bcb8661 relation: 10.51227/ojafr.2025.14