@article{eprints1376, title = {EFFECTIVENESS OF Lactobacillus fermentum CMUL-54 AND Lactobacillus fermentum B978 AS PROBIOTIC CANDIDATES PRODUCING MANNANASE, CELLULASE AND PROTEASE ACTIVITIES FOR POULTRY}, month = {March}, year = {2025}, publisher = {Scienceline Publication, Ltd}, journal = {Online Journal of Animal and Feed Research}, number = {2}, author = {Annisa Rahma Iryos and Mirnawati Mirnawati and Harnentis Harnentis and Anifah Srifani and Gusri Yanti}, volume = {15}, pages = {117--125}, abstract = {The present research investigated the potential of Lactobacillus fermentum strains CMUL-54 and B978 as a probiotic candidates with mannanase, cellulase, and protease activities. The materials used in this research included L. fermentum CMUL-54, L. fermentum B978, MRS Broth containing oxgall, and various equipment and chemicals for analyzing probiotic candidates, mannanase, cellulase, and protease activities. This study utilized quantitative analysis conducted using a paired two-sample t-test with ten replications. The results revealed that L. fermentum CMUL-54 could be significantly (P{\ensuremath{<}}0.01) used as a probiotic candidate, showing resistance to temperatures of 42?C (9.9x109{$\pm$}0.71 CFU/ml), gastric pH (72.35{$\pm$}0.80), bile salt resistance (87.69{$\pm$}3.66), and hydrophobicity test to the intestine (92.40{$\pm$}0.30). Lactobacillus fermentum CMUL-54 also exhibited significant inhibitory zones against lactic acid bacteria (LAB) and pathogenic bacteria such as Escherichia coli (13.27{$\pm$}0.13mm), Salmonella enteritidis (13.91{$\pm$}0.13mm), Staphylococcus aureus (17.75{$\pm$}0.24mm), high activity mannanase (12.36{$\pm$}0.61U/ml), cellulase (12.42{$\pm$}0.24U/ml) and protease (11.30{$\pm$}0.08U/ml). It is concluded that L. fermentum CMUL-54 exhibited superior probiotic properties compared to L. fermentum B978, thus positioning it as a more promising candidate for improving broiler performance through enhanced digestion and overall health. {\copyright} The Author(s) 2025}, keywords = {Enzyme activity; L. fermentum B978; Lactobacillus fermentum CMUL-54; Probiotics}, url = {http://eprints.science-line.com/id/eprint/1376/} }