relation: http://eprints.science-line.com/id/eprint/1372/ title: ESTIMATION OF CRUDE FIBER CONTENT OF A FEED FROM ITS ADF VALUE WHERE THERE IS NO LABORATORY SERVICE creator: Woldemariam, Getachew ASSEFA subject: SF Animal culture description: Because of the cost and inaccessibility of laboratory facilities, animal feed formulation at the farm level, in many parts of Ethiopia, is based on feed database information. However, nowadays many laboratories are phasing out the Weende crude fiber (CF) method of analysis. The fiber content of feeds available in most feed databases (including the sub-Saharan Africa feeds composition database) are a result of detergent method analysis (NDF, ADF and lignin). However, CF is still used in poultry feed formulation and forage analysis for horses, in addition to the neutral detergent fiber (NDF) fraction for determining fiber in different countries. Since there is a statistically (P<0.01) difference between the CF and acid detergent fiber (ADF) value of a feed, ADF can’t be used directly in place of CF. Therefore, this work aims to formulate a regression equation that could roughly estimate the CF level of a feed from its NDF and ADF values. Considering the strong multicollinearity between NDF and ADF, this study developed separate models for ADF and NDF and compared them based on R2 and Akaike Information Criterion (AIC), and the ADF-based model provided a better fit. The equations 0.79×ADF-0.46, 0.01+0.79×ADF, and 1.37+0.62×ADF have effectively predicted CF for cereal grains and beans, pulses and byproducts, and also oilseed meals and cakes, respectively. For grass forages, the equation 3.38+0.76×ADF, tested on 10 forages, showed potential but remains unreliable due to its R2 value below 0.8. Finally, it is concluded that this approach provides a practical alternative for estimating CF where laboratory services or database information are unavailable. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/. © The Author(s) 2025 publisher: Scienceline Publication date: 2025-03-30 type: Article type: PeerReviewed format: text language: en identifier: http://eprints.science-line.com/id/eprint/1372/1/OJAFR15%282%2979-88%2C2025.pdf identifier: (2025) ESTIMATION OF CRUDE FIBER CONTENT OF A FEED FROM ITS ADF VALUE WHERE THERE IS NO LABORATORY SERVICE. Online Journal of Animal and Feed Research. pp. 79-88. ISSN 2228-7701 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-105003491873&doi=10.51227%2fojafr.2025.10&partnerID=40&md5=aa01a483bcf45eef92084de00df67be9 relation: 10.51227/ojafr.2025.10