eprintid: 1356
rev_number: 7
eprint_status: archive
userid: 5
source: Scopus
dir: disk0/00/00/13/56
datestamp: 2025-12-26 09:09:21
lastmod: 2025-12-26 09:09:21
status_changed: 2025-12-26 09:09:21
type: article
metadata_visibility: show
creators_name: Sewoyo, Palagan Senopati
creators_name: Purwanti, Ni Luh Lasmi
creators_name: Munawaroh, Muhammad
creators_name: Kardena, I Made
creators_name: Astawa, I Nyoman Mantik
creators_id: palagansenopati@gmail.com
title: Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: 7,12-dimethylbenz[a]anthracene, Animal model, Breast cancer, Mammary fat pad, Rat
abstract: Chemical-induced mammary cancer models are widely used to mimic human breast carcinogenesis, with 7,12-dimethylbenz[a]anthracene (DMBA) being a commonly used agent. The oral administration of DMBA frequently results in the formation of tumors at random locations and carries significant risks, including high mortality rates and damage to various organs. To address these issues, this study employed a subcutaneous DMBA administration protocol to induce mammary cancer in rats. A total of twenty-four female Sprague-Dawley rats aged 45-55 days weighing 112-130 g were divided into four groups, including the control group injected with 0.75 mL corn oil (D0), a single dose of DMBA at 80 mg/kg BW (D1), two doses with a one-week interval (D2), and three doses with one-week intervals (D3), all administered via subcutaneous in mammary fat pad. Control groups (D0) did not show any tumor growth. Mammary tumor incidence increased with dosage (D1 33.33%, D2 66.67%, and D3 100%). Histopathological examination revealed the presence of various mammary tumor types without evidence of metastasis in all induced rats. All tumors originated from the injection site, and only a single nodule was observed in each rat. There were no significant differences in tumor grades between the treatment groups, and no mortality was recorded during the study. The D3 group showed the highest tumor incidence over the three-month observation period. These findings suggest that subcutaneous DMBA administration effectively induces mammary cancer in rat models with controlled tumor localization and minimal systemic effects, making it a promising method for experimental breast cancer studies.
date: 2024-12-25
publication: World's Veterinary Journal
volume: 14
number: 4
publisher: Scienceline Publication
pagerange: 490 -497
id_number: 10.54203/scil.2024.wvj56
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85214414945&doi=10.54203%2fscil.2024.wvj56&partnerID=40&md5=0422a5af4f919e892decf9b8b2903233
j_index: scopus
citation:    (2024)     Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats.   World's Veterinary Journal.  490 -497.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/1356/1/WVJ14%284%29%20490-497%2C%202024%20%282%29.pdf