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primary topic: Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats

Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats
type: AcademicArticle
type: Article
abstract: Chemical-induced mammary cancer models are widely used to mimic human breast carcinogenesis, with 7,12-dimethylbenz[a]anthracene (DMBA) being a commonly used agent. The oral administration of DMBA frequently results in the formation of tumors at random locations and carries significant risks, including high mortality rates and damage to various organs. To address these issues, this study employed a subcutaneous DMBA administration protocol to induce mammary cancer in rats. A total of twenty-four female Sprague-Dawley rats aged 45-55 days weighing 112-130 g were divided into four groups, including the control group injected with 0.75 mL corn oil (D0), a single dose of DMBA at 80 mg/kg BW (D1), two doses with a one-week interval (D2), and three doses with one-week intervals (D3), all administered via subcutaneous in mammary fat pad. Control groups (D0) did not show any tumor growth. Mammary tumor incidence increased with dosage (D1 33.33%, D2 66.67%, and D3 100%). Histopathological examination revealed the presence of various mammary tumor types without evidence of metastasis in all induced rats. All tumors originated from the injection site, and only a single nodule was observed in each rat. There were no significant differences in tumor grades between the treatment groups, and no mortality was recorded during the study. The D3 group showed the highest tumor incidence over the three-month observation period. These findings suggest that subcutaneous DMBA administration effectively induces mammary cancer in rat models with controlled tumor localization and minimal systemic effects, making it a promising method for experimental breast cancer studies.
date: 2024-12-25
volume: 14
issue: 4
is part of: World's Veterinary Journal
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats (Text)
has version: Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats (Text)
has published: Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats (Text)
subject: SF Animal culture

Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats (Text)
type: Document
has file: WVJ14(4) 490-497, 2024 (2).pdf
has part: WVJ14(4) 490-497, 2024 (2).pdf

Scienceline Publication
type: Organization

World's Veterinary Journal
type: Collection
issn: 23224568

Ni Luh Lasmi Purwanti
type: Person
given name: Ni Luh Lasmi
family name: Purwanti

I Nyoman Mantik Astawa
type: Person
given name: I Nyoman Mantik
family name: Astawa

I Made Kardena
type: Person
given name: I Made
family name: Kardena

Muhammad Munawaroh
type: Person
given name: Muhammad
family name: Munawaroh

Palagan Senopati Sewoyo
type: Person
given name: Palagan Senopati
family name: Sewoyo

http://eprints.science-line.com/id/eprint/1356/
title: HTML Summary of #1356 <epc:when xmlns:epc="http://eprints.org/ep3/control" test="title">Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats</epc:when>
format: text/html
primary topic: Establishment of Mammary Tumors by Injection of 7,12-Dimethylbenzaanthracene in Mammary Fat Pad of Rats

SF Animal culture
type: Concept