eprintid: 1252 rev_number: 9 eprint_status: archive userid: 5 dir: disk0/00/00/12/52 datestamp: 2025-02-07 19:25:53 lastmod: 2025-02-07 19:25:53 status_changed: 2025-02-07 19:25:53 type: article metadata_visibility: show creators_name: Sri Surya Heryani, Luh Gde creators_name: Werdi Susari, Ni Nyoman creators_name: Merdana, I Made creators_id: surya_heryani@unud.ac.id creators_id: surya_heryani@unud.ac.id creators_id: surya_heryani@unud.ac.id title: Identification of Genetic Diversity of Taro White Cattle Using Microsatellite DNA Markers ispublished: pub subjects: SF divisions: j13 full_text_status: public keywords: Conservation, Genetic diversity, Microsatellite DNA, Taro white cattle abstract: Taro white cattle have a small population found in Taro Village, Tegalalang, and Gianyar, Bali, which must be protected as one of the most valuable biological resources for the Hindu community in Bali. White cattle require conservation efforts, with morphometric characterization and genetics providing the first phases of an animal conservation program. The purpose of this study was to identify the genetic diversity of Taro white cattle based on allele frequency, heterozygosity, and fixation index (FIT) using different microsatellite DNA loci so that it can be used as a study and reference in determining policies for the conservation of Taro white cattle in Indonesia, particularly in Bali. This cross-sectional observational study utilized ETH 185, INRA 035, and INRA 037 microsatellite DNA markers. Polymerase chain reaction amplified a total of 22 Taro white cattle blood samples with an average of 4.33 alleles; the average observed heterozygosity and expected heterozygosity were 0.288 and 0.637, respectively, with an average fixation index value of 0.55. In conclusion, there is a significant deviation from the Hardy-Weinberg equilibrium with the likelihood of inbreeding, as indicated by the Hardy-Weinberg balance. The microsatellite loci used in this study can be further used to evaluate the genetic diversity of Taro white cattle. date: 2024-09-25 publication: World’s Veterinary Journal volume: 14 number: 3 publisher: SCIENCELINE pagerange: 417-423 refereed: TRUE issn: 2322-4568 official_url: https://www.scopus.com/record/display.uri?eid=2-s2.0-85206325093&origin=resultslist&sort=plf-f&src=s&sid=d1cddfd490a93522df08bf7f6d0da4f3&sot=a&sdt=a&s=SOURCE-ID+%2821100842862%29AND%28%28+PUBYEAR+%3D+2024%29+OR+%28+PUBYEAR+%3D+2023%29+OR+%28+PUBYEAR+%3D+2022%29+OR+%28+PUBYEAR+%3D+2021%29%29AND+%28%28++DOCTYPE+%28+ar+%29++OR++DOCTYPE+%28+re+%29++OR++DOCTYPE+%28+cp+%29++OR++DOCTYPE+%28+dp+%29++OR++DOCTYPE+%28+ch+%29++%29+AND+NOT+DOCTYPE+%28+undefined+%29%29+AND++NOT+PUBSTAGE+%28+aip+%29++&sl=272&sessionSearchId=d1cddfd490a93522df08bf7f6d0da4f3&relpos=7 j_index: scopus citation: (2024) Identification of Genetic Diversity of Taro White Cattle Using Microsatellite DNA Markers. World’s Veterinary Journal. pp. 417-423. ISSN 2322-4568 document_url: http://eprints.science-line.com/id/eprint/1252/1/WVJ14%283%29%20417-423%2C%202024.pdf document_url: http://eprints.science-line.com/id/eprint/1252/2/WVJ14%283%29%20417-423%2C%202024.pdf