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primary topic: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination

Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination
type: AcademicArticle
type: Article
abstract: Necrotic enteritis causes significant losses in the global poultry industry, necessitating accurate diagnosis for effective intervention. This study aimed to develop a diagnostic tool for detecting necrotic enteritis in poultry based on the presence of Clostridium perfringens (C. perfringens) Alpha-toxin in poultry feces. The reagent of the detection kit was developed by conjugation of IgG against C. perfringens toxin and Staphylococcus cells containing protein A. The IgG antibody was derived from an 8-month-old thin-tailed male sheep immunized with purified 2 ml of C. perfringens Alpha-toxin. Sensitivity assays were carried out to determine the detection limit, while Escherichia coli (E. coli) and Salmonella enteritidis (S. enteritidis) were used to identify specificity. A purified Alpha-toxin with a protein concentration of 2.8 mg/ml and a specific molecular weight of 43 kDa was successfully obtained. A strong reaction of the hyperimmune antibody (IgG) was also detected in the thin-tailed male sheep serum. The developed rapid detection kit in this study indicated C. perfringens Alpha-toxin with a lower concentration (12 ng/ml). Agglutination reactions could differentiate positive control from negative without significant cross-reactivity towards other bacteria (S. enteritidis and E. coli). © The Author(s) 2024
date: 2024-03-25
volume: 14
issue: 1
is part of: World's Veterinary Journal
status: peerReviewed
status: published
author list: authors
type: EPrint
type: ArticleEPrint
is part of: repository
has document: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Text)
has version: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Text)
has published: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Text)
has document: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Other)
subject: SF Animal culture

Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Text)
type: Document
has file: WVJ14(1)8-14,March25,2024.pdf
has part: WVJ14(1)8-14,March25,2024.pdf

Scienceline Publication, Ltd
type: Organization

World's Veterinary Journal
type: Collection
issn: 23224568

M.U.E. Sanam
type: Person
given name: M.U.E.
family name: Sanam

A. Amaliah
type: Person
given name: A.
family name: Amaliah

V.A. Rahmi
type: Person
given name: V.A.
family name: Rahmi

R.S. Kurnia
type: Person
given name: R.S.
family name: Kurnia

A. Soebandrio
type: Person
given name: A.
family name: Soebandrio

R.W. Hakim
type: Person
given name: R.W.
family name: Hakim

C.M.H. Nugroho
type: Person
given name: C.M.H.
family name: Nugroho

A. Indrawati
type: Person
given name: A.
family name: Indrawati

M.A. Putra
type: Person
given name: M.A.
family name: Putra

O.S.M. Silaen
type: Person
given name: O.S.M.
family name: Silaen

. Safika
type: Person
given name: .
family name: Safika

Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Other)
type: Document
is version of: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Text)
is volatile version of: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Text)
is index codes version of: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination (Text)
has file: indexcodes.txt
has part: indexcodes.txt

http://eprints.science-line.com/id/eprint/1213/
title: HTML Summary of #1213 <epc:when xmlns:epc="http://eprints.org/ep3/control" test="title">Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination</epc:when>
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primary topic: Development of Rapid Detection Kit for Necrotic Enteritis Disease in Poultry using Protein A Agglutination

SF Animal culture
type: Concept