%T Evaluation of Stored Whole Blood and Monitoring the Health of Dogs After Transfusion Using Fresh Whole Blood, Stored Whole Blood, and Packed Red Blood Cells
%N 2
%X Blood products have been widely used in emergencies and treatment, necessitating optimal storage conditions to maintain quality. The current study aimed to evaluate the blood quality during storage, transfusion effectiveness, and reactions during and after transfusion in dogs. Five Greyhounds, including three males and two females aged 2.5 years old, and with 25-30 kg bodyweight, were selected and randomly labeled N1, N2, N3, N4, and N5. Fresh whole blood, stored whole blood, and packed red blood cells from the samples dogs were used for transfusion in the study. The investigated parameters were total protein (TP), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), mean corpuscular volume (MCV), total carbon dioxide (tCO2), creatine kinase (CK), creatinine (CREA), blood urea nitrogen (BUN), glucose (GLU), white blood cells (WBC), red blood cells (RBC), hematocrit (HCT), plaletes (PLT), calcium (Ca), phosphorus (P), chloride (Cl), manganese (Mg), sodium (Na), and potassium (K). The results indicated that all parameters of stored blood samples were in the normal range during 28 days of storage in a refrigerator at 2-4°C. However, some parameters (TP, AST, ALT, ALP, LDH, MCV, tCO2, and K) increased, while others (CK, CREA, BUN, GLU, WBC, RBC, HCT, PLT, Ca, P, Cl, Mg, and Na) decreased during the storage period, especially Ca, P, and Na were below the normal range. All dogs indicated no reactions during and 5 hours after transfusion. However, dogs had symptoms of inappetence and mild diarrhea in 1-2 days after transfusion. Dogs received fresh whole blood recovered on day 3, while dogs of the stored blood recipient group recovered on day 4. By day 5, all dogs were healthy with no abnormal signs. The findings indicated the presence of hematological and biochemical alterations in stored blood, highlighting the importance of considering transfusion of stored blood for patients with critical medical conditions. © Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
%O Faculty of Animal Science and Veterinary Medicine, Nong Lam University - Ho Chi Minh City, Region 6th, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 71308, Viet Nam; An Viet Veterinary Service Company Limited, Ho Chi Minh City, Viet Nam
%J World's Veterinary Journal
%I Scienceline Publication
%L eprints1192
%P 220-227
%D 2024
%R 10.54203/scil.2024.wvj27
%V 14
%A T.T. Nguyen
%A H.T.Q. Nguyen
%A K.N. Dinh
%K alanine aminotransferase; alkaline phosphatase; aspartate aminotransferase; calcium; carbon dioxide; chloride; creatine kinase; creatinine; glucose; lactate dehydrogenase; manganese; phosphorus; potassium; protein; sodium, animal experiment; Article; blood; blood storage; blood transfusion; blood transfusion reaction; controlled study; dog; erythrocyte concentrate; erythrocyte count; female; fresh whole blood; greyhound; hematocrit; leukocyte count; male; mean corpuscular volume; monitoring; nonhuman; platelet count; storage temperature; stored whole blood; urea nitrogen blood level