eprintid: 1190
rev_number: 7
eprint_status: archive
userid: 5
dir: disk0/00/00/11/90
datestamp: 2025-02-08 02:51:23
lastmod: 2025-02-08 02:51:23
status_changed: 2025-02-08 02:51:23
type: article
metadata_visibility: show
creators_name: Paramanandi, D.A.
creators_name: Rukyat, H.
creators_name: Kristianingrum, Y.P.
creators_name: Widyarini, S.
title: Efficacy of Avocado Oil Cream in Wound Repair: Macroscopic Analysis, Inflammatory Cells Count, and Collagen Density
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: avocado oil cream; collagen; unclassified drug; wound healing promoting agent, animal tissue; Article; cell count; clinical effectiveness; controlled study; ddY mouse; drug effect; drug efficacy; drug mechanism; drug response; female; histopathology; mouse; nonhuman; wound healing
note: Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia; Laboratory of Veterinary Histology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia; Department of Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
abstract: The wound healing process goes through a series of complex stages that are mutually continuous, namely inflammation, proliferation, and maturation. Following wound formation and the release of proinflammatory cytokines and growth factors, inflammation occurs immediately after the hemostasis phase. Proliferation occurs when products processed by the growth factors are present. The final stage, maturation, is portrayed by the plan of the extracellular network. This study utilized oil from avocado fruit (Persea americana), which contains linoleic and oleic acid content that supports skin tissue repair. The study aimed to explore the effects of using avocado oil cream on the area of wound healing, the number of inflammatory cells, and the collagen density. Twenty-four female mice, aged eight weeks, were used as experimental animals by making excision wounds using a 4 mm biopsy punch on the dorsal skin on the left and right sides. The mice were divided into four groups based on the percentage of avocado oil in the topical cream included Group K (control, topical cream without avocado oil), Group P1 (5 topical avocado oil cream), Group P2 (10 topical avocado oil cream), and Group P3 (15 topical avocado oil cream). Macroscopic examination of the wounds was conducted daily on days 3, 6, and 9 after topical cream treatment using a digital caliper. A total of 48 skin tissue samples were collected from days 3, 6, and 9 after cream application, which were then processed for histopathology evaluations using hematoxylin-eosin staining and Masson's Trichrome staining. Hematoxylin-eosin staining was used to count the inflammatory cells, and Masson's Trichrome staining was employed to assess collagen density. The results revealed that avocado oil had a great impact on wound closure after 9 days of 15 avocado oil cream treatment, reducing the inflammatory cells after 3-6 days of 10 avocado oil cream therapy, and increasing collagen density after 9 days of 15 avocado oil cream application, as compared to the control, non-avocado oil cream group. Avocado oil can help close wounds, reduced the number of inflammatory cells, and increased collagen density when used in topical pharmaceutical formulations. Avocado oil cream may, therefore, be considered a viable option for wound repair treatment. © The Author(s) 2024.
date: 2024-09-25
publication: World's Veterinary Journal
volume: 14
number: 3
publisher: Scienceline Publication, Ltd
pagerange: 380-388
id_number: 10.54203/scil.2024.wvj45
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85206287113&doi=10.54203%2fscil.2024.wvj45&partnerID=40&md5=375244babe924ad7c2b37906a7cac178
j_index: scopus
citation:    (2024)     Efficacy of Avocado Oil Cream in Wound Repair: Macroscopic Analysis, Inflammatory Cells Count, and Collagen Density.   World's Veterinary Journal.  pp. 380-388.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/1190/1/WVJ14%283%29%20380-388%2C%202024.pdf