eprintid: 1179
rev_number: 7
eprint_status: archive
userid: 5
dir: disk0/00/00/11/79
datestamp: 2025-02-08 03:49:40
lastmod: 2025-02-08 03:49:40
status_changed: 2025-02-08 03:49:40
type: article
metadata_visibility: show
creators_name: Heryani, L.G.S.S.
creators_name: Susari, N.N.W.
creators_name: Yusmalinda, N.L.A.
creators_name: Merdana, I.M.
title: Identification of Genetic Diversity of Taro White Cattle Using Microsatellite DNA Markers
ispublished: pub
subjects: SF
divisions: j13
full_text_status: public
keywords: microsatellite DNA, adult; Article; blood sampling; cattle breed; chromosome 10; chromosome 16; chromosome 17; controlled study; cross-sectional study; DNA determination; DNA marker; female; fixation index; gene amplification; gene frequency; gene locus; genetic conservation; genetic parameters; genetic variability; Hardy Weinberg equilibrium; heterozygosity; inbreeding; Indonesia; male; nonhuman; observational study; polymerase chain reaction; Taro white cattle
note: Department of Veterinary Basic Science, Faculty of Veterinary Medicine, Udayana University, Bali, Denpasar, 80234, Indonesia; Yayasan Biodiversitas Indonesia (BIONESIA), Bali, Denpasar, 80234, Indonesia; Departemnt of Veterinary Pharmacy and Pharmacology, Faculty of Veterinary Medicine, Udayana University, Bali, Denpasar, 80234, Indonesia
abstract: Taro white cattle have a small population found in Taro Village, Tegalalang, and Gianyar, Bali, which must be protected as one of the most valuable biological resources for the Hindu community in Bali. White cattle require conservation efforts, with morphometric characterization and genetics providing the first phases of an animal conservation program. The purpose of this study was to identify the genetic diversity of Taro white cattle based on allele frequency, heterozygosity, and fixation index (FIT) using different microsatellite DNA loci so that it can be used as a study and reference in determining policies for the conservation of Taro white cattle in Indonesia, particularly in Bali. This cross-sectional observational study utilized ETH 185, INRA 035, and INRA 037 microsatellite DNA markers. Polymerase chain reaction amplified a total of 22 Taro white cattle blood samples with an average of 4.33 alleles; the average observed heterozygosity and expected heterozygosity were 0.288 and 0.637, respectively, with an average fixation index value of 0.55. In conclusion, there is a significant deviation from the Hardy-Weinberg equilibrium with the likelihood of inbreeding, as indicated by the Hardy-Weinberg balance. The microsatellite loci used in this study can be further used to evaluate the genetic diversity of Taro white cattle. © The Author(s) 2024
date: 2024-09-25
publication: World's Veterinary Journal
volume: 14
number: 3
publisher: Scienceline Publication, Ltd
pagerange: 417-423
id_number: 10.54203/scil.2024.wvj49
refereed: TRUE
issn: 2322-4568
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85206325093&doi=10.54203%2fscil.2024.wvj49&partnerID=40&md5=41a16dfdea00daac0adaf24970ad195b
j_index: scopus
citation:    (2024)     Identification of Genetic Diversity of Taro White Cattle Using Microsatellite DNA Markers.   World's Veterinary Journal.  pp. 417-423.  ISSN 2322-4568   
document_url: http://eprints.science-line.com/id/eprint/1179/1/WVJ14%283%29%20417-423%2C%202024.pdf