relation: http://eprints.science-line.com/id/eprint/1122/ title: THE POLYMORPHISM OF LEPTIN AND THYROGLOBULIN GENES IN LAI SIND AND LAI BRAHMAN CATTLE creator: CHAU, H.L.Q. creator: TRA, T.T.T. creator: HUONG, D.T. creator: HANG, L.T.T. creator: DUNG, D.V. subject: SF Animal culture description: The aim of this study was to investigate the single nucleotide polymorphisms (SNPs) present in intron 2 region of leptin (LEP) and 5’ untranslated region of thyroglobulin (TG5) genes in Lai Sind and Lai Brahman cattle populations raised in the Central Vietnam. For each cattle group, fifty hair root samples were collected and extracted genomic DNA. The LEP/Sau3AI and TG5/PsuI gene polymorphisms were analyzed using PCR-RFLP technique. The results showed that the SNPs of LEP/Sau3AI and TG5/PsuI were found in the both cattle groups. Three LEP/Sau3AI genotypes were detected, including LEPAA, LEPAB and LEPBB. All of the investigated cattle carried TG5CT genotype. The Hardy-Weinberg equilibrium was reached in the both cattle populations for LEP/Sau3AI, but not for TG5/PsuI. The polymorphisms at these two loci were moderate in the both cattle populations. It can be concluded that the SNPs LEP/Sau3AI and TG5/PsuI can be used as genetic markers for molecular selection in these cattle groups. A selection program is needed to increase the frequency of TG5T allele in Lai Sind and Lai Brahman cattle groups to improve beef marbling score. © The Author(s) 2024 publisher: Scienceline Publication date: 2024-05-25 type: Article type: PeerReviewed format: text language: en identifier: http://eprints.science-line.com/id/eprint/1122/1/OJAFR14%283%29165-170%2C2024.pdf identifier: (2024) THE POLYMORPHISM OF LEPTIN AND THYROGLOBULIN GENES IN LAI SIND AND LAI BRAHMAN CATTLE. Online Journal of Animal and Feed Research. pp. 165-170. ISSN 22287701 relation: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85203205339&doi=10.51227%2fojafr.2024.20&partnerID=40&md5=030daf280e963e93a9c7996398c81ed5 relation: 10.51227/ojafr.2024.20