eprintid: 1119
rev_number: 7
eprint_status: archive
userid: 2
dir: disk0/00/00/11/19
datestamp: 2024-12-16 15:42:30
lastmod: 2024-12-16 15:42:30
status_changed: 2024-12-16 15:42:30
type: article
metadata_visibility: show
creators_name: Widiyastuti, T.
creators_name: Rahayu, S.
creators_name: Suryapratama, W.
creators_name: Suhartati, F.M.
title: NUTRIENT PROFILE, PROTEASE AND CELLULASE ACTIVITIES OF PROTEIN EXTRACTED FROM BLACK SOLDIER FLY (Hermetia illucens) LARVAE REARED ON VARIOUS SUBSTRATES
ispublished: pub
subjects: SF
divisions: j11
full_text_status: public
keywords: Amino acid; Chemical profile; Enzymes; Fatty acid; Maggot
note: Faculty of Animal Science, University of Jenderal Soedirman, Jl. Dr. Soeparno, Karangwangkal, Purwokerto, Indonesia
abstract: The Black Soldier Fly (BSF; Hermetia illucens) larvae are recognized for their ability to convert diverse organic materials into protein-rich biomass, depending on the substrate they consume. The composition of these substrates can significantly impact the nutrient profile and enzyme activities of the resulting maggot protein extract (MPE). Therefore, this exploratory research aimed to assess the nutrient content, protease, and cellulase activity of MPE obtained from BSF maggots reared on different substrates, with a specific focus on substrates A (comprising restaurant waste and rejected milk), B (layer manure), and C (kitchen waste). The results showed that maggot meal from layer manure had the highest protein content (45.36) and the lowest fat content (18.44). Amino acids in maggot meal contained high levels of glutamic acid, aspartic acid, alanine, valine, leucine, and isoleucine. Lauric acids were found in maggot meal from kitchen waste (33.79), layer manure (32.18), and restaurant waste and rejected milk (22.94). Maggot meal from layer manure had the highest oleic acid content (15.13). The protein concentration of MPE from various substrates ranged from 0.56 to 0.601 mg/ml (at 60 w/v ammonium sulfate saturation) and 0.555 to 0.609 mg/ml (at 70 ammonium sulfate saturation). The protease activity of MPE from layer manure substrates exhibited optimum activity and stability in neutral to alkaline pH, with activity levels of 0.748 U/mg at pH 7.0 and pH 11.0 (at 60 w/v ammonium sulfate saturation) and 0.774 units/mg at 70 w/v ammonium sulfate saturation. The highest cellulase activity was found in MPE from kitchen waste, which remained stable at pH 5.0–11.0. In general, maggots from different substrate sources exhibited distinct nutrient profiles and enzyme activities. Protein extract from maggots grown in layer manure showed the most suitable nutrient profile for use as an alternative source of protein feed and protease enzymes. © (2024), (Scienceline Publication). All rights reserved.
date: 2024-09-30
publication: Online Journal of Animal and Feed Research
volume: 14
number: 5
publisher: Scienceline Publication, Ltd
pagerange: 309-320
id_number: 10.51227/OJAFR.2024.36
refereed: TRUE
issn: 2228-7701
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85208467905&doi=10.51227%2fOJAFR.2024.36&partnerID=40&md5=166c6745d75eab727ed8b6ece183fb67
j_index: scopus
citation:    (2024)     NUTRIENT PROFILE, PROTEASE AND CELLULASE ACTIVITIES OF PROTEIN EXTRACTED FROM BLACK SOLDIER FLY (Hermetia illucens) LARVAE REARED ON VARIOUS SUBSTRATES.   Online Journal of Animal and Feed Research.  pp. 309-320.  ISSN 2228-7701   
document_url: http://eprints.science-line.com/id/eprint/1119/1/OJAFR14%285%29309-320%2C2024.pdf