eprintid: 1111
rev_number: 7
eprint_status: archive
userid: 2
source: Scopus
dir: disk0/00/00/11/11
datestamp: 2025-01-07 18:20:33
lastmod: 2025-01-07 18:20:33
status_changed: 2025-01-07 18:20:33
type: article
metadata_visibility: show
creators_name: Ondho, Y.S.
creators_name: Sutiyono, S.
creators_name: Setiatin, E.T.
creators_name: Sutopo, S.
creators_name: Kurnianto, E.
creators_name: Samsudewa, D.
creators_name: Lestari, D.A.
creators_name: Setiaji, A.
title: ASPIRATION, SLICING, AND FLUSHING MEDIUM TECHNIQUES IN COLLECTING OOCYTES OF SHEEP: SEARCHING FOR THE BEST METHOD
ispublished: pub
subjects: SF
divisions: j11
full_text_status: public
keywords: Ewes; Oocytes; Ovaries; Reproductive techniques; Sheep breeding
note: Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, Central Java, Semarang, 50275, Indonesia
abstract: The aim of this study was to compare the effect of the techniques of aspiration, slicing, and flushing medium in collecting oocytes on the quantity and quality of oocytes, the average time used for collecting oocytes per ovary, and the volume of the medium used. The material utilized was 274 ovaries from ewes aged 2.5 to 3.5 years and body weight ranging between 25 and 35 kg. This study used a Completely Randomized Design consists of 3 treatments of techniques were aspiration, slicing, and flushing medium. The parameters measured included the average quantity, quality, and weight of oocytes per ovary (right/left), the effect of the techniques of aspiration, slicing, and flushing medium on the quantity and quality of oocytes, and the efficiency of use of medium and time spent to collect oocytes by using aspiration, slicing, and flushing medium techniques. Data were analyzed by one-way analysis of variance. The results showed that the aspiration technique collected the highest percentage (P<0.01) of oocytes quality A (38.49) compared to the slicing technique (17.93), and the flushing medium technique (11.71). In terms of time, the aspiration technique was the fastest (8–10 minutes) compared to the slicing technique (10–12 minutes), and flushing medium technique (13–15 minutes); meanwhile, the aspiration technique was the most efficient technique (1–2 ml) compared to slicing technique (3–5 ml) and flushing medium technique (6–10 ml). In conclusion, the aspiration technique is the best one for oocyte collection from sheep ovaries. This technique proves to be efficient in terms of quantity and quality of the oocytes collected, time to perform, and medium to use. © The Author(s) 2024
date: 2024-03-30
publication: Online Journal of Animal and Feed Research
volume: 14
number: 2
publisher: Scienceline Publication
pagerange: 144-149
id_number: 10.51227/ojafr.2024.17
refereed: TRUE
issn: 22287701
official_url: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85191772124&doi=10.51227%2fojafr.2024.17&partnerID=40&md5=167bd75534708a56c2201bcd7d150c06
j_index: scopus
citation:    (2024)     ASPIRATION, SLICING, AND FLUSHING MEDIUM TECHNIQUES IN COLLECTING OOCYTES OF SHEEP: SEARCHING FOR THE BEST METHOD.   Online Journal of Animal and Feed Research.  pp. 144-149.  ISSN 22287701   
document_url: http://eprints.science-line.com/id/eprint/1111/1/OJAFR14%282%29144-149%2C2024.pdf