%L eprints101
%X Many broiler chickens farms in Diyala Province, Iraq, have shown clinical signs of infectious bursal disease virus (IBDV) in broiler chickens, including depression of birds, petechial hemorrhage in muscles of the thigh, and swollen bursa. Therefore, in the current study, necropsy samples (liver, spleen, bursa, and kidney) were collected from the mentioned broiler chickens. The samples were then subjected to conventional RT-PCR using specific primers to the VP2 gene. The results showed that five out of seven collected samples were positive to IBDV, and the RT-PCR produced a fragment of 260 bp. Furthermore, four tissue bursa samples were sent to AniCon Labor GmbH- Germany by using (FTA- card including four spots) for detection of IBDV by real-time RT-PCR using VP1 and VP2 genes to distinguish the pathogenic virulent strain of IBDV from non-virulent IBDV strains and to make the phylogenetic tree. Results showed that duplex RT-PCR generated a fragment of 690bp for VP1 gene and 700bp for VP2 genes of detected IBDV. Two out of 4 samples from suspected flocks were found positive with prevalence results of 50% for intermediated and non-virulent IBDV strains (IBDV nvv), and 50% (2 samples) were very virulent (vvIBDV). The threshold Cycle (Ct) value for RT-PCR for two isolates on bursa tissue ranged from 16.6 to 25.7. One strain was recorded in NCBI with the accession number of (MW8883071). Phylogenetic analysis of detected and sequenced IBDV indicated that the local studied virus was closely related to 710- Jordan isolate (accession number MF142560.1) and to the isolate 267-Jordan (MF 142517.1) with a higher identity reach of (99.2%).
%V 11
%T Molecular Detection of Infectious Bursal Disease Virus in Broiler Chickens of Diyala Province, Iraq
%I Scienceline Publication, Ltd
%A A. K. Al-Azzawi
%A A. T. Nasser
%A K. S. Al-Ajeeli
%D 2021
%J Journal of World's Poultry Research
%K Broiler chickens, Infectious bursal disease virus, RT-PCR
%R 10.36380/JWPR.2021.52
%P 439-445
%N 4